A Novel Genetic Method For Generating Gain-Of-Function Mutations
- 詳細技術說明
- Scientists at UCSD have discovered methods and compositions for generating and using novel overexpression activity alleles of a gene in any organism, especially Drosophilia. Such alleles may be utilized in screening assays, and used to generate dominant-negative forms of bacterial toxins. The method of the present invention provides advantages in efficiency and speed over current methods and is not based on assumptions relating to protein domains or functions, and thus can provide recovery of mutants in unbiased fashion. Although the method was developed for use in Drosophila, it should be applicable to yeast, C. elegans, Arabidopsis and mammals. The method can be used to generate novel mutations in human genes as expressed in test systems to lead to functional determinations and identification of medically relevant partner genes. Dominant-negative forms of bacterial toxins can be designed to interfere with and neutralize natural toxins thereby providing a protective product.
- *Abstract
-
Pharmacological research is hampered by the labor-intensive and extremely lengthy identification and systematic characterization procedures for new therapeutic compounds. Thousands of individual compounds are conventionally screened for a desired biological therapeutic benefit. Less than 1 in 10,000 of the synthetic compounds screened have ultimately been approved by the Food and Drug Administration with costs approximating more than $200 million per drug put into service. A method is needed that could generate dominant alleles of a wide range of genes using various mutagens to provide insight to the function and mechanism of action of novel genes. This approach should be of particular utility in investigating the function of human disease genes, which have no known functional motifs but have homologues in Drosophila. Furthermore, the method should be applicable to any organism in which it is possible to misexpress transgenic constructs at high levels in a conditional fashion. as developed for use in Drosophila, it should be applicable to yeast, C. elegans, Arabidopsis and mammals. The method can be used to generate novel mutations in human genes as expressed in test systems to lead to functional determinations and identification of medically relevant partner genes. An analogous method when applied to Arabidopsis can be used to isolate and identify plant genes controlling agriculturally desirable traits.
- *IP Issue Date
- Mar 20, 2007
- *Principal Investigation
-
Name: Ethan Bier
Department:
Name: Annabel Guichard
Department:
Name: Shaila Srinivasan
Department:
- 附加資料
- Patent Number: US7193126B2
Application Number: US2002263367A
Inventor: Bier, Ethan | Guichard, Annabel | Srinivasan, Shaila
Priority Date: 1 Oct 2001
Priority Number: US7193126B2
Application Date: 1 Oct 2002
Publication Date: 20 Mar 2007
IPC Current: A01K0067033 | A61K0031255 | C12N001585
US Class: 800021 | 800013 | 800022
Assignee Applicant: The Regents of the University of California
Title: Method for generating overexpression of alleles in genes of unknown function
Usefulness: Method for generating overexpression of alleles in genes of unknown function
Summary: M1 is useful in generating in an organism a novel overexpression activity (NOVA) allele of a gene of unknown function (claimed).
Novelty: Generating in an organism a novel overexpression activity (NOVA) allele of a gene of unknown function by mutagenizing male organisms carrying the transgene-x and screening progeny for new NOVA dominant phenotypes
- 主要類別
- 生物醫學
- 細分類別
- DNA /基因工程
- 申請號碼
- 7193126
- 其他
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Additional Technologies by these Inventors
Tech ID/UC Case
22709/2001-107-0
Related Cases
2001-107-0
- 國家/地區
- 美國
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