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Protein Stabilization In The Presence Of Organic Solvents

*Abstract: This UCB innovation has particular applications in biofuel production. It increases tolerance of microorganisms to toxic agents, such as solvents. Therefore, it is very valuable in increasing production of solvents from solvent-generating microorganisms. The method allows the engineering a microorganism of interest to express a heterologous heat-shock protein/chaperone, e.g., Group II chaperoning or a prefoldin such as γ-prefoldin, where the heterologous protein is from an extremophile, such as an archaean. While the research organism is E. coli, the methods can be applicable to other organisms, such as yeasts

*Principal Investigation: Name: Douglas Clark
Department:

Name: Acchalee Jiemjit
Department:

Name: Pongpan Laksanalamai
Department:

Name: Frank Robb
Department:

Name: Timothy Whitehead
Department:

附加資料: Patent Number: US8685729B2
Application Number: US2009528620A
Inventor: Clark, Douglas S. | Whitehead, Timothy | Robb, Frank T. | Laksanalamai, Pongpan | Jiemjit, Anchalee
Priority Date: 26 Feb 2007
Priority Number: US8685729B2
Application Date: 12 Nov 2010
Publication Date: 1 Apr 2014
IPC Current: C12N000500 | C07H002104 | C07K000100 | C12N000100 | C12N000120 | C12N001574 | C12P000706 | C12P000716 | C12P002106 | C12Q000102
US Class: 435375 | 435029 | 4350691 | 435160 | 435161 | 435243 | 43525233 | 43525422 | 435471 | 530350 | 5360237
Assignee Applicant: The Regents of the University of California | University of Maryland Baltimore,Baltimore
Title: Heterologous expression of extremophile heat shock proteins and chaperones in microorganisms to increase tolerance to toxic compounds
Usefulness: Heterologous expression of extremophile heat shock proteins and chaperones in microorganisms to increase tolerance to toxic compounds
Summary: The methods are useful for increasing the tolerance of a microorganism to a toxic agent; for obtaining a toxic compound from the microorganism; and for increasing the resistance of the microorganism to lethal heat (all claimed).

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