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Reagents To Study The Structure And Function Of The Prototype Map Kinase Scaffold Protein, Ste5

*Abstract: This invention makes available plasmids designed to express derivatives of Saccharomyces cerevisiae Ste5 protein tagged with an N-terminal in-frame (His)6 tract and a c-Myc epitope recognized by the monoclonal antibody, 9E10, and /or fused in-frame at either its N- or C-terminus to the Aequoria victoria Green Fluorescent Protein (GFP), or mutant derivatives of Ste5 in these contexts.
References:
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Inouye et al. 1997. Science 278:103-6
Inouye et al. 1997. Genetics 147:479-92
Sette et al. 2000. Mol Biol. Cell 11:4033-49
Bardwell et al. 2001. J. Biol. Chem. 276:10374-86
Kunzler et al. 2001. Genetics 157:1089-105

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