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New technique for high speed, three-dimensional imaging

技術優勢: 3D volumetric images are generated at higher speeds than existing approaches, which are much more complex. 3D information is obtained without the use of multiple objective lenses, cameras or the need for sample mounting, translation or rotation. Unlike existing orthogonal light-sheet methods, this technology can image both cleared and intact scattering samples such as the rodent brain. The system design has almost no moving parts, uses inexpensive components, and could be portable, compact and even minaturized.The technology does not rely on computationally expensive model-based image reconstruction.Opportunity for development of proprietary 4D dynamic image analysis and visualization packages.Patent information: Issued Patent US 8,619,237

技術應用: 3D live-cell imaging: capturing both cell motion and signaling dynamics in single cells, ensembles, intact and engineered tissues.Imaging small living organisms: drosophila (fruit fly), zebra-fish, c-elegans etc. for neuroscience, developmental, cardiovascular, genetics, disease and drug discovery research. In-vivo mammalian brain imaging: cranial-window based imaging of hemodynamics, intrinsic contrast, voltage and calcium sensitive dyes and fluorescent proteins. Human clinical imaging: intrasurgical, retinal, dermal and endoscopic imaging of superficial tissues. Exploiting the power of GCaMP and new transgenic proteins with sensitivity to voltage and other molecular and signaling targets.Fluid dynamics: detecting subsurface flows and convection and turbulent flow patterns Industrial materials: analyzing transparent and semi-transparent plastics, metal alloys and ceramics for defects. High-speed profilometry of reflective, scattering samples. Extensions to image Raman scattering, second harmonic generation, two and three-photon effects. Extension to superresolution via STED or structured light implementations.

*Inquiry: Teresa FazioColumbia Technology VenturesTel: (212) 854-8444Email: TechTransfer@columbia.edu

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