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Anti-Beta-Catenin (15B8) Monoclonal Antibody

詳細技術說明

Project #:  D1995-12bAntigen Specificity: Beta – cateninClone: 15B8Specificity: Detects bands of ~97kDa by Western blot.Host species: MouseReactive species: Human, Cow, Dog, BirdsDescription:The 15B8 monoclonal antibody reacts with mammals and birds beta-catenin, one member of a family of catenins, which are intracellular proteins that interact with cadherins to mediate cellular adhesion. More specifically, beta-catenin binds to the cytoplasmic tail of E-cadherin. In addition, this molecule is a component of the canonical Wnt signaling pathway, a key player in such functions as embryonic development, the induction of cell polarity, and the determination of cell fate.  In the absence of Wnt binding its receptor, beta-catenin is phosphorylated and resides in the cytoplasm where it is eventually targeted for degradation by ubiquitination. Upon Wnt binding, beta-catenin becomes dephosphorylated, translocates to the nucleus, and modulates gene expression in partnership with the transcription factors T cell factor (TCF) and lymphocyte enhancer binding factor (LEF). Expression of beta-catenin is found in a wide variety of non-immune and immune tissues, including thymocytes and T and B lymphocytes. The Wnt & beta-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and hematopoietic stem cells.Applications: Western blot, Immunoprecipitation, Immunofluorescence, Flow cytometry

*Abstract

*Principal Investigation

Name: Margaret Wheelock, Professor

Department: Biology

Name: James Wahl

Department: Department of Oral Biology

Name: Keith Johnson, Professor

Department: Oral Biology

國家/地區

美國