Blackleg resistance gene
- 詳細技術說明
- The purpose of this invention is to control blackleg disease in Brassica napus canola/rapeseed. This disease caused by Leptosphaeria maculans is one of the most serious diseases in canola/rapeseed production globally. The most effective method to control this disease in canola/rapeseed is to develop blackleg disease resistant cultivars.The problem this invention solves is knowledge of the gene underlying resistance. Using a known blackleg resistance gene leads to the effective and efficient development of new B. napus canola/rapeseed cultivars with strong and durable blackleg disease resistance. In this patent, the cloning and functional confirmation of a blackleg disease gene is described and the sequence including the structural disease resistance gene and its functional promoter region and downstream region is included. The function of the disease resistance gene was definitively confirmed through complementary plant transformation. This blackleg disease resistance gene can be used in conventional breeding through marker assisted selection or through the introduction of the blackleg resistance gene by plant transformation. Additionally, the sequence of this blackleg resistance gene makes it possible to search other homologous disease resistance genes in different Brassica species.
- *Abstract
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The canola cultivar 'Surpass 400' was released as a blackleg resistant cultivar containing one or more blackleg resistance genes. This study focused on the mapping and cloning of a blackleg resistance gene from this cultivar through map-based cloning strategy. A consensus map was developed using SRAP (Sequence related amplified polymorphism) markers and a double haploid (DH) population developed from a cross of 'Westar' and 'Zhongyou 821'. F2 and BC2 and BC3 individuals of the 'Westar' × 'Surpass 400' cross were used to follow the segregation of disease resistance. One Mendelian gene controlled the disease resistance to blackleg as shown by trait segregation. Starting with an anchoring marker on the ultra-density map, different markers including SNP, SSR and SCAR markers were developed and used to screen over 10,000 BC3 individuals to narrow down the blackleg disease resistance gene in a 15-kb region. One gene candidate found in the 15-kb region was used to do complementary transformation. After introducing the candidate gene into the blackleg disease susceptible canola cultivar 'Westar', this cultivar became equally blackleg resistant to the cultivar 'Surpass 400'.
Applications
The blackleg resistance gene can be used in three major applications: 1) developing molecular markers within 5 cM of each side of the gene for transferring the resistance gene, gene pyramiding and eliminating unwanted flanking regions; 2) introducing the blackleg disease resistance gene into susceptible breeding lines or cultivars to improve the blackleg disease resistance or into blackleg resistant line or cultivars to increase the level of blackleg disease resistance; 3) finding new blackleg disease resistance genes or new blackleg disease resistant sources through homologous searches.
- *Inquiry
- Jody Dexter,Ph.DTechnology ManagerPh:+1(204)474.8966Email: jody.dexter@umanitoba.ca
- 附加資料
- Patent Number: WO2011044694A1
Application Number: WO2010CA1635A
Inventor: LI, Genyi
Priority Date: 13 Oct 2009
Priority Number: WO2011044694A1
Application Date: 13 Oct 2010
Publication Date: 21 Apr 2011
IPC Current: C12N001529 | A01H000500 | C07K0014415 | C12N000510 | C12N001582
Assignee Applicant: University of Manitoba
Title: BLACKLEG RESISTANCE GENE | GÈNE DE RÉSISTANCE À LA JAMBE NOIRE
Usefulness: BLACKLEG RESISTANCE GENE | GÈNE DE RÉSISTANCE À LA JAMBE NOIRE
Summary: The nucleic acid molecule and protein molecule are useful for inhibiting L. maculans . The protein molecule and composition are useful for providing a plant with resistance to blackleg disease (all claimed).
Novelty: New blackleg resistance gene, useful for inhibiting Leptosphaeria maculans, and for providing a plant with resistance to blackleg disease
- 主要類別
- 生物醫學
- 細分類別
- DNA /基因工程
- 國家/地區
- 美國
