Media.Player

kHz Frame Rate Confocal Imaging

總結

Purdue University researchers have designed a confocal microscope capable of capturing quantitative images with a frame rate on the scale of kHz, which is faster than the 30 Hz for current microscopy techniques. As opposed to the rectangular scan pattern used in other microscopes, the Purdue researchers' instrument captures pixels along a Lissajous curve whose geometry can be tuned by the user. This scan pattern requires much less time to capture an image. By coupling this scan pattern with image processing software, the potential for this approach is unlocked. For any given frame of a movie, the dark spots within the scan pattern can be statistically interpolated from the scanned pixels and the movement of the image over time. If a high-resolution still image is preferred, then individual frames are combined, like those in a panoramic photograph, to assemble the high-resolution image. In this way, both low-resolution movies and high-resolution stills can be captured from the same data set. This combination of Lissajous scan-pattern and software processing shows promise for faster detection of microscopic processes and is flexible enough to be extended for long-distance applications like lidar.

技術優勢

Much faster frame rate than current confocal microscopesRaw data can be used to generate high-resolution still imageGeneration of a high-frame rate, lower-resolution movie

技術應用

Microscopy applications where movement distortion is a problemMicroscope use in exotic environmentsLidar telescopesScanned laser imaging

詳細技術說明

Garth SimpsonSimpson Laboratory for Nonlinear OpticsPurdue Chemistry

*Abstract

*Background

Confocal microscopy give researchers the ability to produce high-resolution, three-dimensional images of subjects as diverse as crystals, cells, and even the fluorescence of single molecules. Although confocal microscopes are a common tool used to generate static images, current instruments cannot capture fast processes such as chemical reactions. Further, confocal microscopy is difficult to use outside of the lab because the instrument must be isolated from vibrations while a sample is scanned.

*IP Issue Date

None

*IP Type

Utility

*Stage of Development

Proof of Concept

*Web Links

Purdue Office of Technology CommercializationPurdueInnovation and EntrepreneurshipGarth SimpsonSimpson Laboratory for Nonlinear OpticsPurdue Chemistry

國家

United States

申請號碼

None

國家/地區

美國