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B-18 A fish model that is transparent throughout life

技術優勢

Has already been used as an experimental model fish in many areas of biological research including pathology, toxicology and embryology Has smaller genome and shorter generation time than another popular model fish, the zebrafish Changes can be easily observed in its internal body structure from outside due to its highly transparent body

技術應用

Medical research to observe disease progression and healing process by developing medicines Test fish to observe the effectiveness of water pollutants

詳細技術說明

The resulting fish is a vertebrate model with a transparent body in the adult stage, as well as during its embryonic stages. The main internal organs such as the brain and heart in living adult fish are visible to the naked eye or with a simple stereoscopic microscope. Another feature of see-though medaka is the ability to observe specific organs by producing targeted luminescence through the use of green fluorescent protein. The fish remains healthy and fertile.

*Abstract

Medaka (Oryzias latipes, aka Japanese killifish) is widely used as a simple and useful experimental animal due to its small body size (2 - 4cm), a short term for sexual maturation (two months), a wide distribution, a small genome size (about 1/5 of mammalsΓÇÖ), and its sex determination (X & Y chromosomes.) The embryo is especially useful to observe its internal structure due to its transparency. The adult medaka, however, has a darker body due to its four types of pigment cells in the skin, and its color makes internal organs difficult to observe.Researchers at Nagoya University have successfully removed the pigments from the adult fish body and generated a see-though adult medaka.

附加資料

Patent Number: US6737559B2
Application Number: US2001814047A
Inventor: Wakamatsu, Yuko | Ozato, Kenjiro | Tanaka, Minoru | Kinoshita, Masato
Priority Date: 8 Jun 2000
Priority Number: US6737559B2
Application Date: 22 Mar 2001
Publication Date: 18 May 2004
IPC Current: A01K0067027 | C07K0014435 | C12N000510 | C12N001509 | C12N001585
US Class: 800020 | 800022
Assignee Applicant: Wakamatsu Yuko | Ozato Kenjiro
Title: See-through medaka
Usefulness: See-through medaka
Summary: (I) is useful as a research resource for biology, medical sciences and fishery sciences, as an experimental animal for testing toxic chemicals, as a teaching material for science education in schools, and as an aquarium fish.
Novelty: Novel transparent medaka deficient in iridophores, melanophores, xanthophores and leucophores, useful as research resource, experimental animal for testing toxic chemicals, as teaching material and as aquarium fish

主要類別

農業

細分類別

動物

國家/地區

美國