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A-47 Photo-responsive oligonucleotide

技術優勢

● The cis-form of S-Azo is more stable than Azo.● The wavelength at absorption maximum (λmax) becomes larger by using S-Azo, and the DNA duplex photoisomerization occurs in visible lights. .● 400nm light accelerates to form cis-form and 450nm to form trans-form.● The larger ⊿Tm makes the hybridization of oligonucleotides more efficient.

技術應用

ΓùÅ Reversibly controllable DNA-enzyme and Ribozyme (A light-switching for m-RNA splicing) .ΓùÅ Photo-responsive DNA nanomachineΓùÅ Gene expression control by light (A light-controlled transcription by T7 RNA polymerase)ΓùÅ Nano capsule which opens and closes by light

詳細技術說明

None

*Abstract

Researchers have revealed that it is possible to control visible-light-photoisomerization in DNA duplex by using azobenzene derivatives containing sulfur atoms, instead of regular azobenzene. It was additionally discovered that this S-azobenzene enlarges a difference (⊿Tm) between melting temperatures in DNA duplex and makes hybridization more efficient. Furthermore, cis-isoform of S-azobenzene (S-Azo) is more stable in heat than regular azobenzene (Azo).

附加資料

Inventor: ASANUMA Hiroyuki | LIANG Xingguo | KASHIDA Hiromu | NISHIOKA Hidenori | FUJII Taiga | ISHIKAWA Teruchika
Priority Number: WO2012029434A1
IPC Current: C07H002100 | C12N001509
Assignee Applicant: National University Corporation Nagoya University
Title: OLIGONUCLEOTIDE AND USE THEREOF | OLIGONUCLÉOTIDE ET UTILISATION DE CELUI-CI
Usefulness: OLIGONUCLEOTIDE AND USE THEREOF | OLIGONUCLÉOTIDE ET UTILISATION DE CELUI-CI
Summary: The oligonucleotide is useful for as agent for controlling formation and dissociation of double chain (claimed).
Novelty: New oligonucleotide comprising azobenzene derivative used as agent for controlling formation and dissociation of double chain, comprises azobenzene derivative

主要類別

化工/材料

細分類別

化工/材料應用

國家/地區

美國