Media.Player

Novel Adjuvant for Increasing Effectiveness of Vaccines

技術優勢

Investigators have tested the combination of adjuvants (alum and MPL) in a mice model of Influenza A infection. Mice primed with nucleoprotein of influenza A (NP) and both adjuvant lost less weight and quickly regained their original weight in contrast to mice primed with NP/protein and either adjuvant.

技術應用

A combination of two adjuvants, such as alum and MPL, with an internal viral protein can be used to induce CD8, (killer) T cells to join antibodies in response to viral infection. CD8 T cell epitopes are much less variable and thus a vaccine designed to activate protective CD8 T cells has the potential to protect against yearly and newly emerging pandemic viral subtypes. This new approach could be applicable to infectious disease such as the flu and malaria.

詳細技術說明

Most current vaccines, including those against influenza, act via the generation of specific antibodies that can either neutralize or otherwise inactivate the pathogen. These vaccines induce the production of antibodies against viral surface proteins to prevent viral cellular entry. However, as far as influenza is concerned, these viral surface proteins tend to mutate over time and as a result a new vaccine against influenza must be developed every year. To avoid this problem, the ideal vaccine would be pan-specific across strains of influenza virus.Targeting CD8 T cell mediated immunity could be the right strategy to reach this goal. The portions of influenza virus that are recognized by cytotoxic CD8 T cells are much less variable than those recognized by antibodies. Thus a vaccine designed to activate CD8 T cells has the potential to protect against yearly and newly emerging pandemic viral subtypes.

*Abstract

Dr. MarrackΓÇÖs laboratory at National Jewish Health has discovered how to prime a second arm of the immune system to boost the effectiveness of influenza vaccines. They demonstrated that the combination of two adjuvants (alum and monophosphoryl lipid A, MPL), already approved for patient use, with a viral nuclear protein can maintain long-lived memory CD8 T cells and protect mice from influenza viral challenge.

*Principal Investigation

Name: John Kappler, Professor

Department: Integrated Department of Immunology

Name: Philippa Marrack, Professor

Department: Integrated Department of Immunology

附加資料

Inventor: Jin, Shouguang | Bichsel, Candace
Priority Number: US8617888B2
IPC Current: C12N000500 | C07H002104 | C07K000100 | C07K001400 | C07K001700 | C12N000502
US Class: 435377 | 530350 | 5360235
Assignee Applicant: University of Florida Research Foundation Inc.inesville
Title: Bacterial mediated delivery of nuclear protein into pluripotent and differentiated cells
Usefulness: Bacterial mediated delivery of nuclear protein into pluripotent and differentiated cells
Summary: The method and tool are used for delivering one or more proteins into a cell (claimed).
Novelty: Delivering proteins into a cell, comprises incubating a cell with a Pseudomonas deletion mutant harboring the proteins, collecting the cells, and sorting the cells to isolate and quantify a subpopulation expressing the proteins

主要類別

生物醫學

細分類別

DNA /基因工程

國家/地區

美國