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Dual Emission Fluorescent Compounds

總結: Dual emission fluorescence probes for ultrasensitive ratiometric sensing

技術優勢: Highly accurate radiometric measurements.
Stop/go indicator or two colour readout.
Room temperature fluorescence and low photobleaching.
Freely cell permeable.
Many analogues and variations possible.
Stable.
Simple synthesis

技術應用: Cell sorting and imaging
Super resolution microscopy.
Environmental sensing.
Protein detection.
Optoelectronic devices.

詳細技術說明: A 'self-calibrating' (ratiometric) fluorophores that display dual emission by combining excited state intramolecular proton transfer (ESIPT) with normal intramolecular charge transfer (ICT). Such fluorophores have a unique sensitivity to subtle variations in the local environment that are independent of the probe concentration and other variables.
We have invented new chemical entities (NCEs) that emit fluorescence at two different frequencies (eg cyan and red) in response to the local environment. This new class of two-colour fluorophores is based on the chromophore found inside the Green Fluorescent Protein (GFP)

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