miR-195 Antagomir Treatment of Sepsis
MicroRNA’s (miRNA’s) are small non-coding RNA molecules encoded by eukaryotic nuclear DNA, on average 22 nucleotides long, that function in transcriptional and post-transcriptional regulation of gene expression via base-pairing with complementary sequences within mRNA molecules, usually resulting in gene silencing via translational repression or target degradation. Aberrant expression of miRNA’s has been implicated in numerous disease states including sepsis (Wang et. al. PLoS ONE, 2012, 7(6):e38885). Abrogation of inflammatory mediators and apoptosis to protect cells/tissues/organs is a promising therapeutic strategy for sepsis.
MicroRNA’s（miRNA’s）是真核DNA编码的非编码RNA小分子，平均有22个核甘酸的长度，通过与mRNA分子的互补序列碱基配对基因表达进行转录和转录后调控，通常通过转译抑制或靶向降解引起基因沉默。很多疾病状态，包括败血症涉及miRNA’s的异常表达（Wang等人，PLoS ONE，2012年，7（6）：e38885）。通过废除炎症介质和细胞凋亡保护细胞/组织/器官是很有潜力的败血症治疗策略。
Our researchers have now demonstrated in two different animal models of sepsis(LPS-induced; FIP(feces-in-peritoneum)-induced)that inhibition of miR-195 not only prevents apoptosis, but also significantly reduces inflammation and prevents multiple organ dysfunctions(liver, kidney, lung)during sepsis. The combined results indicate that inhibition of miR-195isa promising therapeutic approach for sepsis.
我们的研究人员正在用两种不同的败血症动物模型（LPS诱发模型；FIP（猫传染性腹膜炎）诱发模型）中验证抑制miR-195不仅能防止细胞凋亡，还能显著减轻败血症的炎症，防止多器官衰竭（肝、肾、肺）。综合结果表明抑制miR-195是很有潜力的败血症治疗方法。
Decreased apoptosis of endothelial cells (as indicated by TUNEL and caspase-3assays) were evident in liver, kidney and lung protecting the vasculature and attenuating microvascular dysfunction. Inflammation was significantly decreased in liver, kidney and lung as evident through decreased levels of myeloperoxidase (MPO) activity and inflammatory mediators (iNOS; TNF-alpha). Functional tests indicate significant protection and preservation of liver (ALT & AST) and kidney (BUN) organ function during sepsis.
能显著减少肝、肾、肺的内皮细胞凋亡（TUNEL化验和caspase-3化验结果），保护脉管系统，缓解微血管功能障碍。髓过氧物酶（MPO）活性水平的降低和炎症介质（iNOS；TNF-alpha）的减少表明肝、肾、肺炎症明显减轻。功能测试表明能在败血症中起到显著的肝（ALT和AST）肾（BUN）器官功能保护作用。
MiR-15 family member inhibition (i.e. miR-195) reduces apoptosis, inflammation and micro vascular dysfunction apoptosis to protect and preserve multiple organ function (liver, kidney, lung) during sepsis.
MiR-15家族成员抑制剂（即miR-195）能减少细胞凋亡，减轻炎症，缓解微血管功能障碍细胞凋亡，在败血症中保护多器官（肝、肾、肺）功能。
Finished two different animal models of sepsis (LPS-induced; FIP(feces-in-peritoneum)-induced)
已完成两种败血症动物模型（LPS诱发模型；FIP（猫传染性腹膜炎）诱发模型）试验
技术售让
生物技术/制药/医疗
药械组合
