TWO-WAY, PORTABLE RIBOSWITCH MEDIATED GENE EXPRESSION CONTROL DEVICE
A regulatable gene expression construct comprising a nucleic acid molecule comprising a two-way riboswitch operably linked to a target sequence. Also provided is a library screening strategy for efficient creation of target-specific riboswitches. A theophylline-repressible and IPTG-inducible riboswitch device achieves portable control of gene expression control in a `two-way` manner. The default state of target genes is ON; the targets are switched off by adding theophylline, and switched back to the ON-state by adding IPTG without changing growth medium. The riboswitch device regulates gene expression in a portable, adjustable, and two-way manner with a variety of scientific and biotechnological applications.
Patent Number: US9222093B2
Application Number: US13534774A
Inventor: Huang, Jian-Dong | Jin, Ye
Priority Date: 30 Jun 2011
Priority Number: US9222093B2
Application Date: 27 Jun 2012
Publication Date: 29 Dec 2015
IPC Current: C12N0015113 | C12N001563 | C12N001567 | C12N001572 | C12N0015115
Assignee Applicant: The University of Hong Kong
Title: Two-way, portable riboswitch mediated gene expression control device
Usefulness: Two-way, portable riboswitch mediated gene expression control device
Summary: The gene expression control device is useful for studying gene function, and in medical field, preferably in gene therapy and biotechnology.
Novelty: Gene expression control device useful for studying gene function, comprises chloramphenical resistance gene linked to theophylline-specific aptamer fragment, lactose (lac) I gene, and native lacI-lacZ intergenic region
生物醫學
DNA /基因工程
美國
