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Using a Novel High-throughput Yeast Cell-Based Screening Platform to Identify HMG-CoA Reductase Inhibitors from Natural Products

總結: It introduces methods of screening, identifying and isolating HMG-CoAR inhibitors. In particular, said method is useful for screening natural raw products. It also provides potent HMG-CoAR inhibitors for treatment and prevention of diseases and conditions associated with elevated cholesterol and/or blood lipid levels

技術優勢: High efficient, high-throughput and reproducible method for screening HMG-CoAR inhibitors

技術應用: Treat and prevent diseases and conditions associated with elevated cholesterol and/or blood lipid levels

詳細技術說明: It provides a novel screening method using yeast cells and a kit to identify HMG-CoAR inhibitors. Also it provides a yeast cell-based activity-guided fractionation assay screening platform to identify and isolate HMG-CoAR inhibitors from natural products. Furthermore, it provides composition for treating and preventing diseases and conditions associated with elevated cholesterol and blood lipid level, such as hypercholesterolemia and hyperlipidemic. The present composition for treating and preventing diseases and conditions associated with elevated cholesterol and blood lipid level comprises an effective amount of an extract of Gynostemma pentaphyllum (Gp).

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