Method For Constructing And Modifying Large Dna Molecules
This Invention Provides A Method For Combining Overlapping Dna Molecules Comprising: (A) Providing First And Second Dna Fragments, The First Having A Region Homologous To A Region In The Second; (B) Tagging The First Dna Fragment With A Selectable Marker; (C) Cloning The First Dna Sequence Into A Retrieval Vector To Form A Dna-Vector Complex; (D) Linearizing The Dna-Vector Complex; And (E) Inserting The First Dna Fragment From The Dna-Vector Complex Into The Second Dna Fragment Using Homologous Recombination To Form A Combined Dna Molecule; And (F) Removing The Selectable Marker, Thereby Generating A Combined Dna Molecule. The Invention Further Provides A Vector For Retrieving And Inserting A Selected Dna Molecule Into A Target Dna Molecule.
Patent Number: US7781190B2
Application Number: US2004892579A
Inventor: Huang, Jian-Dong | Zhang, Xin-Mei | Tanner, Julian Alexander
Priority Date: 24 Jul 2003
Priority Number: US7781190B2
Application Date: 16 Jul 2004
Publication Date: 24 Aug 2010
IPC Current: C12N001564 | C12N001510 | C12N001570
US Class: 4350914 | 435471 | 435478 | 435488
Assignee Applicant: The University of Hong Kong
Title: Method for constructing and modifying large DNA molecules
Usefulness: Method for constructing and modifying large DNA molecules
Summary: The method is useful for bacterial artificial chromosome manipulation to aid in future studies of gene function in the post-genomic era. The method is useful for engineering any large DNA fragments or inserting large DNA fragments into the Escherichia coli chromosome. It is also useful for constructing and modifying much larger DNA molecules such as human artificial chromosomes.
Novelty: Combining overlapping DNA molecules, useful for human artificial chromosome manipulation for gene function study, by inserting a first DNA fragment from a DNA-vector complex into a second DNA fragment by homologous recombination
化工/材料
金屬材料
香港
