High-Throughput Diagnostic Assay For The Human Virus Causing Severe Acute Respiratory Syndrome (Sars)
The Present Invention Relates To A High-Throughput Diagnostic Assay For The Virus Causing Severe Acute Respiratory Syndrome (Sars) In Humans ("Hsars Virus"). In Particular, The Invention Relates To A High-Throughput Reverse Transcription-Pcr Diagnostic Test For Sars Associated Coronavirus (Sars-Cov). The Present Assay Is A Rapid, Reliable Assay Which Can Be Used For Diagnosis And Monitoring The Spread Of Sars And Is Based On The Nucleotide Sequences Of The N (Nucleocapsid)-Gene Of The Hsars Virus. The Present Method Eliminates False Negative Results And Provides Increased Sensitivity For The Assay. The Invention Also Discloses The S (Spike)-Gene Of The Hsars Virus. The Invention Further Relates To The Deduced Amino Acid Sequences Of The N-Gene And S-Gene Products Of The Hsars Virus And To The Use Of The N-Gene And S-Gene Products In Diagnostic Methods.; The Invention Further Encompasses Diagnostic Assays And Kits Comprising Antibodies Generated Against The N-Gene Or S-Gene Product.
Patent Number: US7547512B2
Application Number: US2004807807A
Inventor: Peiris, Joseph S. M. | Yuen, Kwok Yung | Poon, Lit Man | Guan, Yi | Chan, Kwok Hung | Nicholls, John M. | Leung, Frederick C.
Priority Date: 24 Mar 2003
Priority Number: US7547512B2
Application Date: 24 Mar 2004
Publication Date: 16 Jun 2009
IPC Current: C12Q000168 | A61P001100 | A61P003114 | C12N001550 | C12Q000100 | C12Q000170
US Class: 435005 | 435006 | 435004 | 43500612 | 53602372 | 53602432 | 53602433
Assignee Applicant: The University of Hong Kong
Title: High-throughput diagnostic assay for the human virus causing severe acute respiratory syndrome (SARS)
Usefulness: High-throughput diagnostic assay for the human virus causing severe acute respiratory syndrome (SARS)
Summary: The nucleic acid molecule and polypeptide, methods, and kit are useful diagnosing and monitoring the spread of SARS. The vaccine and pharmaceutical compositions are useful for treating SARS.
Novelty: New nucleic acid molecule encoding nucleocapsid- or spike-gene protein of a human Severe Acute Respiratory Syndrome (hSARS) virus, useful for diagnosing and treating SARS
生物醫學
病原
香港
