Using transposable elements in rice
- 详细技术说明
- Transposableelements are DNA sequences capable of hopping from one spot in a genome toanother. These DNA elements may be used to introduce foreign DNA sequences intoan organism for applications including genetic engineering, mutant straincreation, and gene mapping. The rice genome is one source of transposableelements for plant genetics, with a total of 6% being comprised of miniatureinverted-repeat transposable elements (MITEs). MITEs are derivatives of DNAtransposons, which use a “cut and paste” method of insertion. MITEs have beenidentified in rice, flowering plants, and other animal genomes. logy provides amethod to genetically engineer rice to render it with, for instance, highernutritional qualities.
- *Abstract
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Organisms: Oryza sativa (Asian rice), Oryza glaberrima (African rice) and other Poaceae
Technology Description
New activelytransposing MITEs have been identified from the rice genome. These are themPing/Pong family of DNA transposable elements and Pong family of transposases.MITEs aid in the introduction of transposable elements into eukaryotic cells,insertion of DNA sequences, mutant strain creation, and expression ofrecombinant proteins. These MITEs can be mobilized by multiple transposases andincorporated into expression vectors. The transposases and transposableelements may be optimized and used for genetic engineering of transgenic plantsand seeds. The invention enables and comprises new DNA transposons/transposableelements for plant genetic manipulation, creation of transgenic plants with atleast 2-fold increased rates of transposition, development of expressionvectors with at least 2-fold increased transposition rates for rapid geneticmanipulation and is suitable for stable introduction of DNA into eukaryoticcells.
References and Intellectual Property
Jiang N, Feschotte C, Zhang X, Wessler SR: Using rice to understand the originand amplification of miniature inverted repeat transposable elements (MITEs). CurrOpin Plant Biol. 2004 Apr;7(2):115-9
Zhang X, Jiang N, Feschotte C, Wessler SR: PIF-and Pong-like transposable elements: distribution, evolution and relationshipwith Tourist-like miniature inverted-repeat transposable elements. Genetics. 2004 Feb;166(2):971-86
Feschotte C, Osterlund MT, Peeler R, WesslerSR.: DNA-binding specificity of rice mariner-like transposases and interactionswith Stowaway MITEs. NucleicAcids Res. 2005 Apr 14;33(7):2153-65
- Yang G, Zhang F, HancockCN, Wessler SR: Transposition of the rice miniature inverted repeattransposable element mPing in Arabidopsis thaliana. ProcNatl Acad Sci U S A. 2007 Jun26;104(26):10962-7
- 国家/地区
- 美国
