Mouse monoclonal antibodies against antigenic domains on phytochrome from plants (angiosperms). (11 lines)
- 详细技术说明
- Applications Phytochrome is a photomorphogenically active pigment thatmodulates plant growth and development with respect to incident light intensityand wavelength distribution. It exists in two forms: an inactive, red-absorbingform (Pr), and an active far-red-absorbing form (Pfr). When either absorbslight, it is photoconverted to the other. Phytochrome is a dimeric,water-soluble, relatively labile chromoprotein with similar, if not identical,monomers of about 124 kDa each. It is also a relatively low abundance protein,even under the best of conditions. Genetic manipulation of phytochromeexpression in plants leads to plants requiring less light and able to divertmore energy to the production of fruits and seeds. For its physicochemicalcharacterization, it has therefore been difficult to utilize techniques thatrequire large quantities of highly purified protein. Consequently, indirectmethods for elucidating its structure/function relationships are especiallyimportant. Antibodies Oat-8, Oat-13, Oat-16, Oat-22, Oat-25, Oat-28 and Pea-25were developed to study and characterize phytochromes and, more importantly,map and probe specific epitopes from Avenasativa L. (both green and etiolated) and applicable to other Poaceae(Gramineae). These are also applicable to other angiosperms.
- *Abstract
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ReagentDescription
Antigen:
Phytochrome
Accession ID:
NCBI Ref.Seq.: NP_037111, Uniprot P28494
Line name (Isotype)
Oat-8 (IgG2b); Oat-9 (IgG1); Oat-13 (IgG); Oat-16 (IgM); Oat-17 (IgG1); Oat-22 (IgG); Oat-23 (N/D); Oat-24(N/D); Oat-25 (IgG); Oat-28 (IgG); Pea-25 (IgG)
Epitopes location
Oat-8 ca. 70 kDa from N-terminus; Oat-13 65 kDa from N-terminus; Oat -16 36kDa from N-terminus and very near the site of chromophore attachment (epitope also undergoes photo-convertion as that for Oat-25); Oat-22 36 kDa from N-terminus; Oat-25 N-terminus of phytochrome (this domain undergoes conformational change when phytochrome is interconverted between the red and far-red absorbing forms); Oat-28 75 kDa from N-terminus; Pea-25 located at 85 kDa from N-terminus and is highly conserved (no photo-conversion)
Note: Oat-8 / Oat-28 and Oat-16 / Oat-22 were shown to be distinct by immunoblot
Molecular Weight:
124 kDa
Reactivity:
Avena. Pissum. Very likely other graminae, fabaceae and angiosperms
Immunogen:
Protein/ peptide
Species Immunized:
Mouse
Buffer:
0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications:
ELISA, WB, IP, Competitive ELISA
References
Cordonnier MM,Smith C, Greppin H, Pratt LH. Production and purification of monoclonalantibodies to Pisum and Avena phytochrome. Planta. 1983Aug;158(4):369-76.
M.M. Cordonnier,H. Greppin and Lee H. Pratt (1986) Identification of a Highly Conserved Domainon Phytochrome from Angiosperms to Algae. Plant Physiol.80, 982-987
Pratt LH,Cordonnier MM, Lagarias JC. Mapping of antigenic domains on phytochrome frometiolated Avena sativa L. by immunoblot analysis of proteolytically derivedpeptides. Arch BiochemBiophys. 1988 Dec;267(2):723-35.
KevinL. Childs, Lee H. Pratt, Page W. Morgan. Genetic Regulation of Development inSorghum bicolor: VI. The ma3R Allele Results in Abnormal PhytochromePhysiology. Plant Physiol.1991 Oct; 97(2): 714-719.
YUKIOSHIMAZAKI, MARIE-MICHELE CORDONNIER, AND LEE H. PRATT : Identification withMonoclonal Antibodies of a Second Antigenic Domain on Avena Phytochrome thatChanges upon Its Photoconversion. PlantPhysiol. 1986. 82, 109-113
- 国家/地区
- 美国
