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Anti C. elegans cyclin E (CYE1, full-length recombinant protein prepared in bacteria), Line 17C8

详细技术说明

Applications  This mouse monoclonal antibody was generated againstfull-length recombinant cyclin E (CYE-1) protein and is specific for Caenorhabditis elegans CYE-1 full-lengthrecombinant protein (prepared in bacteria)and has been validated for usein WB, IF (1:100-300). Cyclin E is a member of the cyclin family of proteins, whichregulates the cell cycle through its activation of cyclin-dependent kinases.Specifically, cyclin E binds and activates the S phase Cdk2. The cyclin E–Cdk2complex promotes the G1 to S phase cell cycle transition. Overexpression ofcyclin E has been implicated in carcinomas among the gastrointestinal tract,including colon or stomach cancer as well as a marker for breast cancers.

*Abstract

ReagentDescription           

Antigen:  rec- C. elegans CYE-1

AccessionID:  NM_001025857.2

MolecularWeight:  60.6 kDa

CloneName:  17C8

Reactivity:  C.elegans / C. briggsae

Immunogen:  Full-length recombinant CYE-1 protein

SpeciesImmunized:  Mouse

PurificationMethod:  Not purified; used as antibodysupernatant

TestedApplications:  WB, IF.

MAbstorage: -80^oC

References

 

Brodigan, T.M.,Liu, J., Park, M., Kipreos, E.T., and Krause, M. 2003. Cyclin E expressionduring development in C. elegans. DevelopmentalBiology 254: 102-115.

Kim, Y., andKipreos, E.T. 2007. The C. elegans replication licensing factor CDT-1 istargeted for degradation by the CUL-4/DDB-1 complex. Molecular and Cellular Biology 27:1394-1406.

The, I.,Ruijtenberg, S., Bouchet, B. P., Cristobal, A., Prinsen, M. B., van Mourik, T.,Koreth, J., Xu, H., Heck, A. J., Akhmanova, A., Cuppen, E., Boxem, M., Munoz,J., & van den Heuvel, S. (2015). Rb and FZR1/Cdh1 determine CDK4/6-cyclin Drequirement in C. elegans and human cancer cells. Nat Commun, 6, 5906.

Fox, P. M.,Vought, V. E., Hanazawa, M., Lee, M. H., Maine, E. M., & Schedl, T. (2011).Cyclin E and CDK-2 regulate proliferative cell fate and cell cycle progressionin the C. elegans germline. Development, 138, 2223-34.

国家/地区

美国