Scalable AAV production and purification by plasmid DNA transfection of HEK293 cells
- 详细技术说明
- Recombinant adeno-associated virus (rAAV) vectors have emerged as one of the most versatile and successful gene therapy delivery vectors. Good Manufacturing Practice (GMP) Production Facility at Nationwide Children's Hospital has developed a process to increase the scale of rAAV production using DNA plasmid based transfection of HEK293 cells. This process allows generation of AAV at a 10-fold larger scale than previously produced in the GMP facility thereby supporting trials that require higher doses of clinical grade vector.
- *Abstract
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Recombinant adeno-associated virus (rAAV) vectors have emerged as one of the most versatile and successful gene therapy delivery vectors. Good Manufacturing Practice (GMP) Production Facility at Nationwide Children's Hospital has developed a process to increase the scale of rAAV production using DNA plasmid based transfection of HEK293 cells. This process allows generation of AAV at a 10-fold larger scale than previously produced in the GMP facility thereby supporting trials that require higher doses of clinical grade vector.
- *IP Issue Date
- None
- *IP Type
- Other Patent
- 国家
- Not Available
- 申请号码
- None
- 国家/地区
- 美国
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