Process For Sorting Dispersed Colloidal Structures
Custom-designed shape-complementary particles allow for greater target specificityDesigns are specified to bind targets while not binding non-targetsCustom-shaped particles can be mass produced using existing methods, including spatially patterned radiation and relief deposition templating.Custom-shaped particles can contain fluorescent dyes and, surface charge, and attached polymers for further more specific detection and separation.
Separating red blood cells from whole bloodOrganelle isolation from lysed cellsSeparation of macromolecules from complex solutionBiomarker isolation and detection
Researchers from the Chemistry and Biochemistry department at UCLA have developed a new method of separating and/or sorting specific target structures from other non-target structures in a complex mixture. These target structures and non-target structures are typically objects having maximum dimensions in the range of a few nanometers to hundreds of micrometers that are dispersed in a complex aqueous solution. This is achieved by using custom-designed shape-complementary colloidal particles to specifically separate target colloidal structures from non-target colloidal structures based on differences in attractive interactions.
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Background New methods for making custom-shaped colloidal particles offer unique opportunities for capturing and separating specific molecular, particulate, and cellular species in soft colloidal materials that contain a complex variety of components. An example of a soft colloidal material is human blood, which can contain a wide variety of proteins, complexes, and cells in an aqueous solution at a well-regulated pH. Among the current challenges in the fields of biomedicine and nanomedicine, it is important to develop methods of efficiently separating different components and cell types in human blood with a high degree of shape and size specificity. Diagnostic methods that rely on detecting very small numbers of abnormal cells in blood are also highly desirable. Separation of small numbers of abnormal cells in a viable state would be a major breakthrough. Related Materials Zhao, Kun, and Thomas G. Mason. "Suppressing and enhancing depletion attractions between surfaces roughened by asperities." Physical review letters 101.14 (2008): 148301 Additional Technologies by these Inventors Tech ID/UC Case 27485/2009-059-0 Related Cases 2009-059-0
Mason TG. Osmotically driven shape-dependent colloidal separations. Phys Rev E Stat Nonlin Soft Matter Phys. 2002;66(6 Pt 1):060402.
Hernandez C.J., Mason T.G. Colloidal Alphabet Soup: Monodisperse Dispersions of Shape-Designed LithoParticles. J. Phys. Chem. C 111 4477-4480 (2007).
Hernandez, Carlos J., Kun Zhao, and Thomas G. Mason. "Pillar-Deposition Particle Templating: A High-Throughput Synthetic Route for Producing LithoParticles." Soft Materials 5.1 (2007): 1-11.
Hernandez, Carlos J., Kun Zhao, and Thomas G. Mason. "Well-Deposition Particle Templating: Rapid Mass-Production of LithoParticles Without Mechanical Imprinting." Soft Materials 5.1 (2007): 13-31.
Zhao, Kun, and Thomas G. Mason. "Directing colloidal self-assembly through roughness-controlled depletion attractions." Physical review letters 99.26 (2007): 268301.
Voeltz, Gia K., and William A. Prinz. "Sheets, ribbons and tubules—how organelles get their shape." Nature Reviews Molecular Cell Biology 8.3 (2007): 258-264.
Gourley, Paul L., and Robert K. Naviaux. "Optical phenotyping of human mitochondria in a biocavity laser." Selected Topics in Quantum Electronics, IEEE Journal of 11.4 (2005): 818-826.
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