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Genome-Wide Gene Expression Profiling, Alternative Splicing Monitoring, and Genotyping with Direct Template Annealing Oligo-Ligation

详细技术说明

Scientists at UC San Diego have discovered sensitive and accurate assays for gene detection, genome-wide gene expression profiling, and alternative splice monitoring with minimal or no target-specific amplification. The technology relates to a novel approach of detecting and quantifying DNA and RNA rearrangement in cells. In particular, the technology is designed to analyze alternative RNA processing from common pre-mRNA precursors. The technique can also be extended to DNA research in detecting chromosome rearrangement and translocation. This invention provides a powerful assay to detect alternative splicing on a large scale and can be applied to discover disease-associated alternative splicing events. This technology could have broad applications in disease classification, diagnosis, and drug screening.

*Abstract

Detecting specific nucleic acids is central to diagnostic medicine and molecular biology research. Gene probe assays play a variety of roles, from identifying infectious organisms—such as bacteria and viruses—to probing the expression of normal and mutant genes. Ideally, a gene probe assay should be sensitive, specific, and easily automatable. The requirement for sensitivity (i.e. low detection limits) has been greatly alleviated by the development of the polymerase chain reaction (PCR) and other amplification technologies that allow researchers to amplify exponentially a specific nucleic acid sequence before analysis. Specificity, however, is problematic for many currently available gene probe assays. The extent of molecular complementarities between probe and target defines the specificity of the interaction. A variation in the concentrations of probes, targets, and salts in the hybridization medium, reaction temperature, and length of the probe may influence the specificity of the probe/target interaction.

*IP Issue Date

Nov 2, 2004

*Principal Investigation

Name: Jian-Bing Fan

Department:

Name: Xiang-Dong Fu

Department:

附加资料

Patent Number: US6812005B2
Application Number: US2001779202A
Inventor: Fan, Jian Bing | Fu, Xiang Dong
Priority Date: 7 Feb 2000
Priority Number: US6812005B2
Application Date: 7 Feb 2001
Publication Date: 2 Nov 2004
IPC Current: C12Q000168
US Class: 4350912 | 43500612 | 4350911 | 436094 | 5360231 | 5360243 | 53602433 | 435006
Assignee Applicant: The Regents of the University of California Jolla | Illumina Inc.n Diego
Title: Nucleic acid detection methods using universal priming
Usefulness: Nucleic acid detection methods using universal priming
Summary: The method is used to identify a nucleotide in a genome sequence (claimed). The method is useful in identifying microorganisms, probing expression of normal and mutant genes, identifying mutant genes e.g. oncogenes, in tissue typing, matching tissue and blood samples in forensic medicine, and for exploring homology among genes from different species.
Novelty: Identifying a nucleotide at a detection position, comprising using a polymerase chain reaction and determining the nucleotide

主要类别

生物医学

细分类别

DNA /基因工程

申请号码

6812005

其他

Intellectual Property Info

See the following U.S. patents.

• 6,812,005 issued 2-November-2004, Nucleic Acid Detection Methods Using Universal Priming.
• 7,361,488 issued 22-April-2008, Nucleic Acid Detection Methods Using Universal Priming.

Additional Technologies by these Inventors

• The Chip-Dasl Technology For Functional Genomics Studies
• Gene Signature-Based Chemical Screening
• Trans-Differentiation Of Fibroblasts Into Functional Neurons

Tech ID/UC Case

20616/2000-095-0

Related Cases

2000-095-0

国家/地区

美国