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The Chip-Dasl Technology For Functional Genomics Studies

详细技术说明

Scientists at UC San Diego have developed a new method, which advances the understanding of DNA binding proteins and allows functional genomics questions to be addressed under a variety of physiological conditions. This method allows selective enrichment of functional DNA elements, such as promoters/enhancers, replication origins, and specifically modified sequences, within any genome of interest followed by signal amplification and detection. This is accomplished by coupling the selective enrichment of the functional DNA elements (by chromatin immunoprecipitation, ChIP) with a signal amplification/detection procedure, (DNA Annealing Selection and Ligation, or DASL) which utilizes microarrays. This novel technology, called ChIP-DASL permits robust analysis using fewer materials compared with to standard procedures. The invention has broad applications in the fields of basic research (gene expression studies), clinical diagnosis, and high throughput pharmaco-genomics screening (drug screening assays).

*Abstract

Transcriptional regulation involves a large number of protein complexes specifically assembled at a given promoter to activate or suppress RNA synthesis. In a specific tissue or cell type, a promoter can be turned on by a sequence of specific recognition events. Transcription factors bind cis-acting regulatory sequences and allow these DNA binding proteins to recruit co-activator complexes that further recruit the core transcription machinery. Similarly, a gene can be turned off by the recruitment of transcription co-repressor complexes through sequence-specific DNA binding proteins during repression-involved chromatin remodeling factors that modify histones. A long-term molecular memory may be established by epigenetic modification of a specific chromatin region(s) via DNA methylation.

*Principal Investigation

Name: Xiang-Dong Fu

Department:

Name: Young-Soo Kwon

Department:

附加资料

Patent Number: US7601492B2
Application Number: US2003561764A
Inventor: Fu, Xiang-Dong | Kwon, Young-Soo
Priority Date: 3 Jul 2003
Priority Number: US7601492B2
Application Date: 2 Aug 2006
Publication Date: 13 Oct 2009
IPC Current: C12Q000168 | C12N000900 | C12N001510 | C12N001511
US Class: 43500612 | 435006 | 43500613
Assignee Applicant: The Regents of the University of California
Title: Genome mapping of functional DNA elements and cellular proteins
Usefulness: Genome mapping of functional DNA elements and cellular proteins
Summary: The method and kit are useful for detecting a polynucleotide-polypeptide interaction domain in a genome of an organism and identifying a region of a genome of a living cell to which a polypeptide of interest binds (claimed). The method is useful for genome mapping of functional DNA elements and cellular proteins.
Novelty: Detecting a polynucleotide-polypeptide interaction domain, useful for genome mapping of functional DNA elements and cellular proteins, comprises performing hybridization methods

主要类别

生物医学

细分类别

DNA /基因工程

其他

Patent Information

The patent application numbers are US 200710059703 and WO 20051007814.

Related Materials

Additional Technologies by these Inventors

• Gene Signature-Based Chemical Screening
• Genome-Wide Gene Expression Profiling, Alternative Splicing Monitoring, and Genotyping with Direct Template Annealing Oligo-Ligation
• Trans-Differentiation Of Fibroblasts Into Functional Neurons

Tech ID/UC Case

19173/2003-244-0

Related Cases

2003-244-0

国家/地区

美国