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Chip-Based Droplet Sorting

技术应用: The invention may be used for monitoring bio-threat agents that contain nucleic acid signatures; biomedical applications (e.g., monitoring infectious diseases and DNA detection); high throughput genetic screening for drug discovery and novel therapeutics; genetic screening for oncology, disease, and personal genomics; forensic purposes; and other research applications (including but not limited to compound discovery, proteomics, and crystallography).

*Abstract: Microfluidic devices are poised to revolutionize environmental, chemical, biological, medical and pharmaceutical detectors and diagnostics. The term “microfluidic devices” loosely describes the new generation of instruments that mix, react, count, fractionate, detect, and characterize samples in a micro-electro-mechanical system (MEMS) circuit manufactured through standard semiconductor lithography techniques. Although a wide array of microfluidic technologies are currently available, novel MEMS fluidic systems are needed as scientists continue to work with smaller sample volumes and desire devices with increased sensitivity and effectiveness.

Researchers at the University of California, Irvine have developed a unique non-contact system for sorting monodisperse water-in-oil emulsion droplets in a microfluidic device. The technology can be coupled to other on-chip processes to increase device efficiency by sorting out un-reacted droplets.

*Principal Investigation: Name: Neil Reginald Beer
Department:

Name: Andrew Hatch
Department:

Name: Abraham Lee
Department:

附加资料: Patent Number: US20120196288A1
Application Number: US13014792A
Inventor: Beer, Neil Reginald
Priority Date: 27 Jan 2011
Priority Number: US20120196288A1
Application Date: 27 Jan 2011
Publication Date: 2 Aug 2012
IPC Current: C12Q000168 | C12M000134 | G01N003300
US Class: 43500612 | 4220681 | 4350061 | 4352872
Assignee Applicant: Lawrence Livermore National Security LLC,Livermore
Title: Chip-Based Droplet Sorting
Usefulness: Chip-Based Droplet Sorting
Summary: The apparatus is useful for sorting droplets on chip, where the droplets are monodisperse water-in-oil emulsion droplets, used for identifying, monitoring and detecting bio-threat agents that contain nucleic acid signatures, such as spores, bacteria and virus which causes infectious diseases. The apparatus is also useful for detecting host and viral DNA or RNA in biological fluids for forensic purpose and for screening therapeutic drugs.
Novelty: Apparatus useful for sorting droplets on chip for detecting e.g. viral RNA in sample, comprises main flow channel, carrier fluid in main flow channel, droplet maker, analyzer, waste channel and droplet sorter

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