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Fluorescent Probe for Selective Imaging of Carbon Monoxide in Living Cells Using Palladium-Mediated Carbonylation

技术优势

- Visual (fluorescent) tool for detecting CO (Qualitative and quantitative detection)- Easy to synthesize- Non-toxic- Highly selective for CO

技术应用

- In vitro studies on effects of CO on cells and tissues- Study the effects of CO releasing molecules- Investigation of biochemical pathways

详细技术说明

None

*Abstract

Carbon monoxide (CO) is a well known toxic gas, but emerging research also suggest potentially beneficial effects of temporarily increased levels of CO in attenuating deleterious effects of reactive oxygen species (ROS). To date the study and evaluation of the effects of CO have relied on detecting gross anatomical change of some observable parameter, such as infarct size in the studies of CO on ischemia/reperfusion or offline extracellular measurements using myoglobin as a colorimetric readout. These macroscopic and colorimetric outputs do not provide detailed information about cellular signaling pathways.

Scientists at UC Berkeley have designed, synthesized, and evaluation of a new type of chemical reagent for selective CO detection in living cells by exploiting palladium-mediated carbonylation chemistry. The probe is based on a cyclometallated aryl-palladium compound attached to a BODIPY –type fluorophore that will react with CO in a carbonylation reaction to produce a highly fluorescent amino acid species with concomitant loss of palladium. The probe is capable of displaying an increase in fluorescence in both cuvettes and in live cells. Carbon Monoxide Probe 1 (COP-1) represents a unique first-generation chemical tool that features a robust turn-on response to CO with selectivity over reactive nitrogen, oxygen, and sulfur species. It can be used to detect CO in aqueous buffer and in live-cell specimens, providing a potentially powerful approach for interrogating its chemistry in biological systems.

*IP Issue Date

May 3, 2016

*Principal Investigation

Name: Christopher Chang

Department:

Name: Brian Michel

Department:

申请号码

9329191

其他

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Tech ID/UC Case

23561/2014-010-0

Related Cases

2014-010-0

国家/地区

美国