Media.Player

Surface-enhanced Optical Upconversion Luminescence (SOUL) for Single miRNA Detection

技术应用

Customized SOUL probe targeting specific miRNA for researchers who want to see the expression level of the miRNA of their interests in living single cells.

详细技术说明

None

*Abstract

MicroRNA (miRNA) is a gene expression regulator performing a critical role in epigenetics. Despite its importance in biology, the detection of miRNA at the single-molecule level has not been reported because it is difficult to detect due to its small molecular weight, fast dynamics, and low dehibridization temperature (melting point). Researchers at the University of California, Berkeley, have designed systems for both intracellular detection of single molecule miRNA of living single human cancer cells employing surface-enhanced upconversion using nanoparticles and locked nucleic acid (LNA)­ DNA/LNA-RNA hybridization. They have designed and synthesized gold nanoparticles for non-phototoxic wavelength tuning, and a huge surface enhancement, and finally inject into living single cells of drug-resistant human epithelial lung cancer cells. When they meet the target miRNAs, they will be disassembled which brings a dramatic change of signal measured by a home-built, multi-channel dark field imaging system. 

*Principal Investigation

Name: Kyuwan Lee

Department:

Name: Luke Lee

Department:

其他

Additional Technologies by these Inventors

• Microfluidic Sample Preparation And Impedimetric Detection Of Small Molecules
• Monodisperse Silk Emulsions And Microspheres
• A Porous Microfluidic Spinneret
• Nanophotonic Graphene Transistor
• Self-Powered Blood Coagulation Chip For Inr Value And Hematocrit Determination
• Modular Aptazyme-Mediated Signal Transduction Coupled With Chemical Amplification In A Semi-Quantitative, Colorimetric Diagnostic Assay
• Portable Fluidic Actuation
• Digital Separation For One-Step Hiv Viral Load Monitoring
• Cardiac BioChip for Drug Screening
• Customizable Multi-Organ Biochip
• High-Throughput Rapid Screening Platform For Microalgal Biofuel Applications
• Mobile Molecular Diagnostics System

Tech ID/UC Case

21997/2012-032-0

Related Cases

2012-032-0

国家/地区

美国