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Colloidal Supports For Neuron Culture, Synthetic Networks And Transplantation

技术优势
1). Studying neuronal circuit formation and dynamics:? Neurons can be manipulated genetically and mechanically.? Neurons can be assembled into ordered 2D layers and 3D networks.? Beads coated with chemical guidance can cues direct neuronal growth and promote synapse formation.? Network connectivity can be monitored remotely.2). Conducting neuron-based drug screens:? Individual beads or layered neural networks can be used as platforms for drug screens.3). Exploring therapeutic strategies:? Neurons grown on silica beads could be manipulated and used for cell replacement therapies: ideally, the intact nervous system.
技术应用
1). Studying neuronal circuit formation and dynamics:? Neurons can be manipulated genetically and mechanically.? Neurons can be assembled into ordered 2D layers and 3D networks.? Beads coated with chemical guidance can cues direct neuronal growth and promote synapse formation.? Network connectivity can be monitored remotely.2). Conducting neuron-based drug screens:? Individual beads or layered neural networks can be used as platforms for drug screens.3). Exploring therapeutic strategies:? Neurons grown on silica beads could be manipulated and used for cell replacement therapies: ideally, the intact nervous system.
详细技术说明
None
*Abstract

Scientists at the University of California, Berkeley have developed a method for manipulating highly differentiated neurons in vitro, using silica beads as substrates. The use of silica beads allows for facile assembly of three-dimensional neuronal networks. The method will be a powerful tool for gaining insights into neuronal circuit formation and dynamics, conducting neuron-based drug screens, and exploring therapeutic strategies. The method offers several attractive features, including:

? Silica beads support neuronal growth, maturation, transfection and manipulation.
? Silica beads act as moveable supports for highly differentiated neurons, permitting manipulation of neurons without damaging delicate processes.
? Silica beads spontaneously assemble into hexagonal arrays, facilitating in-vitro circuit construction.
? Silica beads are optically transparent, allowing for non-invasive neuronal stimulation and recording of neuronal activity.

This method will be a powerful tool for:

1). Studying neuronal circuit formation and dynamics:
? Neurons can be manipulated genetically and mechanically.
? Neurons can be assembled into ordered 2D layers and 3D networks.
? Beads coated with chemical guidance can cues direct neuronal growth and promote synapse formation.
? Network connectivity can be monitored remotely.
2). Conducting neuron-based drug screens:
? Individual beads or layered neural networks can be used as platforms for drug screens.
3). Exploring therapeutic strategies:
? Neurons grown on silica beads could be manipulated and used for cell replacement therapies: ideally, the intact nervous system.

*IP Issue Date
Jul 14, 2015
*Principal Investigation

Name: Ehud Yeheskel Isacoff

Department:


Name: Sophie Pautot

Department:

附加资料
Inventor: Gelfand, Erwin W. | Lucas, Joseph J.
Priority Number: US7160681B2
IPC Current: C12Q000168 | G01N003350 | G01N0033574
US Class: 43500614 | 435006 | 43500723
Assignee Applicant: National Jewish Medical and Research Centernver
Title: Method for regulating cell growth and assays related thereto
Usefulness: Method for regulating cell growth and assays related thereto
Summary: The method is used to diagnose and stage tumors, also to monitor their progression or treatment. Compounds that increase activity or expression of cdk6 are useful for treating cancer and lymphoproliferative diseases, or to protect subjects at risk of developing such diseases.
Novelty: Diagnosis, staging and monitoring of tumor growth, by detecting reduced cdk6 activity or expression, also treatment by increasing cdk6 activity
主要类别
诊断/治疗
细分类别
癌症/肿瘤
申请号码
9080148
其他

Related Materials

Colloid-guided assembly of oriented 3D neuronal networks; Sophie Pautot, Claire Wyart & Ehud Y Isacoff; Nat Methods. 2008 Aug;5(8):735-40.


Tech ID/UC Case

17976/2008-121-0


Related Cases

2008-121-0

国家/地区
美国

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