Optical Probes For Biological Hydrogen Peroxide
- 详细技术说明
- None
- *Abstract
-
A new class of fluorescent probes for hydrogen peroxide, known as the Peroxysensor family, has been developed by UC Berkeley researchers. These reagents utilize a boronate deprotection mechanism to provide high selectivity and optical dynamic range for detecting H2O2 in aqueous solution over similar reactive oxygen species (ROS) including superoxide, nitric oxide, tert-butyl hydroperoxide, and hydroxyl radical.
Peroxyresorufin-1 (PR1), Peroxyfluor-1 (PF1), and Peroxyxanthone-1 (PX1) are first-generation probes that respond to H2O2 by an increase in red, green, and blue fluorescence, respectively. The boronate dyes are cell-permeable and can detect micromolar changes in H2O2 concentrations in living cells, including hippocampal neurons, using confocal and two-photon microscopy.
The unique combination of ROS selectivity, membrane permeability, and a range of available excitation/emission colors establishes the value of PR1, PF1, PX1, and related probes for interrogating the physiology and pathology of cellular H2O2. The value of these probes has been demonstrated by measuring changes in intracellular H2O2 within living mammalian cells.
These compounds can be used as probes in a variety of disciplines such as microscopy, enzymology, clinical chemistry, histochemistry, molecular biology and medicine, as well as diagnostic agents in imaging methods, such as magnetic resonance imaging, positron emission tomography, near infrared imaging and the like.
- *IP Issue Date
- Nov 30, 2010
- *Principal Investigation
-
Name: Christopher Chang
Department:
- 附加资料
- Patent Number: US7842823B2
Application Number: US2006588785A
Inventor: Chang, Christopher J.
Priority Date: 27 Oct 2005
Priority Number: US7842823B2
Application Date: 27 Oct 2006
Publication Date: 30 Nov 2010
IPC Current: C07D031180 | C07F000708 | G01N003350
US Class: 549213 | 436068 | 544069
Assignee Applicant: The Regents of the University of California
Title: Fluorogenic probes for reactive oxygen species
Usefulness: Fluorogenic probes for reactive oxygen species
Summary: (I) are useful in methods for assaying a sample for a selected analyte and determining whether a compound alters the activity of an enzyme (claimed). (I) are useful as fluorogenic probes for reactive oxygen species; to detect the presence of and elucidate the complex roles of oxidants, e.g. hydrogen peroxide, in living systems, as small molecules in solution assays or as a label that is conjugated to an analyte or a species that interacts with, and allows detection and/or quantification of an analyte, as probes e.g. in microscopy, enzymology, clinical chemistry, histochemistry, molecular biology and medicine, as therapeutic agents in modalities such as photodynamic therapy and as diagnostic agents in imaging methods such as magnetic resonance imaging, positron emission tomography and near infrared imaging. (I) are also useful as components of optical amplifiers of light and waveguides and to incorporate into inks and dyes for printing currency and other negotiable instruments. The fluorogens of (I) are useful in devices and methods for measuring oxidases e.g. glucose oxidase, amine oxidase and peroxidase.
Novelty: New dioxaborolane substituted xanthone compounds useful as fluorogenic probes for reactive oxygen species, for assaying sample for selected analyte and determining if compound alters activity of enzyme
- 主要类别
- 生物医学
- 细分类别
- 医学影像
- 申请号码
- 7842823
- 其他
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Tech ID/UC Case
17513/2005-077-0
Related Cases
2005-077-0
- 国家/地区
- 美国
