Cell Surface Engineering/chemoselective Ligation Reaction
The reaction can be used to engineer the composition of cell surfaces, as well as cells' interior. Ligations can be performed in aqueous solution under physiological conditions. Unique properties of the reactive partners (abiotic and chemically orthogonal) provide for extreme selectivity of the reaction
Chemoselective ligation reaction can be used as a powerful tool for: Gaining insights into biological processes: Probing intracellular interactions. Delivery of detectable labels, and receptor ligands for binding by a target receptor. Provides ways of engineering receptors for viral mediated gene transfer. Synthesis of peptides and other polymers. Exploring therapeutic strategies: Engineered compounds that can provide for pharmacological activity, or can serve as a target for delivery of other molecules. Provides new ways of labeling cells as targets for cancer therapy. Designing synthetic model surfaces and unique materials for biomedical applications: Provides new ways of adhering cells to nonbiological materials, e.g., medical implants.
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Patent Number: US6570040B2
Application Number: US2001810864A
Inventor: Saxon, Eliana | Bertozzi, Carolyn
Priority Date: 16 Mar 2000
Priority Number: US6570040B2
Application Date: 16 Mar 2001
Publication Date: 27 May 2003
IPC Current: C07F000950 | C07F00096506 | C07F0009655 | C07F00096561 | C07K000102 | C07K000104 | C07K0001107 | C12N000502
US Class: 568017 | 435136 | 435325 | 502162 | 560233 | 568007 | 568008
Assignee Applicant: The Regents of the University of California
Title: Chemoselective ligation
Usefulness: Chemoselective ligation
Summary: For construction of chemical libraries including peptide libraries); modification of surfaces (such as cell surfaces); modification of molecules under physiological conditions (e.g. in a cellular environment); in synthesis of peptides and other polymers; production of modified amino acids and to selectively attach a small molecule to an engineered or semi-synthetic polymer.
Novelty: Chemoselective ligation for construction of peptide library involves reacting an engineered phosphine having an electrophilic trap with azide to form covalent bond between them by intramolecular rearrangement of aza-ylide intermediate
化工/材料
化工/材料应用
6570040
Tech ID/UC Case 16934/2000-060-0 Related Cases 2000-060-0
美国
