Media.Player

Label-Free, Non-Genetic Identification and Sorting of Human Pluripotent Stem Cell Derived Cardiomyocytes

技术优势

Identifying stem cell derived cardiomyocytesSorting for pure populations of stem cell derived cardiomyocytesRemoving pluripotent stem cells from a cardiomyocyte populationAssessing maturity of cardiomyocytesDerivation of clinical grade cardiomyocytesLabel-free (xeno-free)Non-genetic (vector-free)Detection of very immature cardiomyocytesDiscrimination of cells at different stages of maturation

详细技术说明

UC Davis researchers from the NSF Center for Biophotonics and UC Davis Health System have developed a method of identifying and sorting cardiomyocytes derived from human pluripotent stem cells. This method, based on second harmonic generation (SHG) - a nonlinear optical technique, does not require genetic modification of the cell or any exogenous labels to be used, which makes this an attractive technique for obtaining pure populations of cardiomyocytes under xeno- and vector- free conditions most appropriate for clinical and therapeutic use, as well for tissue engineering and drug discovery applications. There are currently no established methods for sorting pur populations of stem cell derived cardiomyocytes. Methods that use fluorescent reporters require the introduction of a reporter vector and result in genetically modified cells, reducing their utility for clinical applications. Other fluorescent-based staining methods have shown to be only applicable for selecting very mature cardiomyocytes. Surface marker based methods require exposing human cells to products of animal origin, which may increase the risk of non-human pathogen transmission and render the cells unsuitable for clinical use. Second harmonic generation (SHG) is a laser-based technique that identifies stem cell derived cardiomyocytes based on the direct detection of myosin bundles, which generates a unique second harmonic signal when excited by intense laser pulses. This signal is specific to the cardiomyocyte phenotype and is absent from undifferentiated stem cells and other non-cardiomyocyte cells that are found in the population following the directed differentiation of stem cells to the cardiac lineage. SHG is able to discriminate cardionmyocytes at different stages of maturation/development, and can detect very immature cells. When integrated into a flow cytometric configuration, non-invasive sorting for pure populations of stem cell derived cardiomyocytes is feasible.

*Abstract

None

*IP Issue Date

Jan 30, 2018

*Principal Investigation

Name: James Chan

Department:

Name: Deborah Lieu

Department:

申请号码

9879224

其他

Additional Technologies by these Inventors

• An Optical System for Parallel Acquisition of Raman Spectra from a 2-Dimensional Laser Beam Array

Tech ID/UC Case

22045/2010-609-0

Related Cases

2010-609-0

国家/地区

美国