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Efficient Production of 14C-Vitamin B12 for Human Microdosing and Other Applications

技术优势: The key novel features of this method are: Microscale but scalable: This method has produced, in a 50mL reaction vessel, enough 14C-B12 to microdose 20 to 40 human subjects. It could, however, easily be scaled up if required. Efficient: The process typically yields purified material in as little as 72 hours. It produces very little radioactive waste. Sensitive Detection: Abundance of 14C in biological materials (blood, urine, saliva) can be determined at high sensitivity in extremely small volumes of biological material using accelerator mass spectrometry. Safety: High sensitivity enables the use of nanocurie doses of 14C-cobalamin in human assays. This resolves safety and disposal issues that were connected to the use of 57Co-Schilling technology. Specificity: The method of microbial synthesis of 14C-labelled cobalamin ensures incorporation of the label into a specific carbon atom of cobalamin only.

详细技术说明: A novel and an efficient method for producing isotopically-labeled forms of vitamin B12 (cobalamins) has been developed by scientists at the University of California, Davis. This invention is coupled to the development of a new set of clinical and diagnostic technologies related to vitamin B12 deficiency and metabolism. Determination of 57Co-B12 is not particularly sensitive or quantitative (i.e., The Schilling Test). In addition, the half-life of 57Co makes it expensive to handle and dispose of properly and radiation dosage exposure to workers, patients, and environment is significant. Our new method overcomes the problems associated with the Schilling Test by using 14C-B12 as the label. Images Appearance of carbon-14 Vitamin B12 in human plasma. Detection is by mass spectrometry

*Abstract: Efficient Production of ¹⁴C-Vitamin B₁₂ for Human Microdosing and Other Applications

*Principal Investigation: Name: Peter Anderson
Department:

Name: Bruce Buchholz
Department:

Name: Colleen Carkeet
Department:

Name: Stephen Dueker
Department:

Name: Ralph Green
Department:

Name: Joshua Miller
Department:

Name: John Roth
Department:

附加资料: Patent Number: US8202507B2
Application Number: US2005234079A
Inventor: Anderson, Peter J. | Dueker, Stephen | Miller, Joshua | Green, Ralph | Roth, John | Carkeet, Colleen | Buchholz, Bruce A.
Priority Date: 23 Sep 2004
Priority Number: US8202507B2
Application Date: 23 Sep 2005
Publication Date: 19 Jun 2012
IPC Current: A61K005100 | A61M003614
US Class: 42400111 | 42400181
Assignee Applicant: The Regents of the University of California
Title: Assay for vitamin B12 absorption and method of making labeled vitamin B12
Usefulness: Assay for vitamin B12 absorption and method of making labeled vitamin B12
Summary: (M1) is useful for labeling vitamin B12 (claimed), which labeled vitamin B12 is useful for studying vitamin B12 metabolism such as plasma clearance, turnover rate, enzyme kinetic measurement, or uptake and distribution of the vitamin; determining absorption of vitamin B12; and studying a number of medical disorders related to abnormal vitamin B12 metabolism.
Novelty: Labeling vitamin B12, by providing facultative anaerobe bacterium having eut operon, and culturing bacterium on media having ethanolamine or propandiol in presence of cobinamide and/or dimethylbenzimidazole, under aerobic conditions

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