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Atomic Force Microscopy-based Platform for Investigating Single Cell Mechanics

技术优势

The main advantage of cell compression method is reliability of data. Individual cell biology is described by statistical parameters rather than by results of single experiment. Cell compression method allows compressing up to 50 cells per hour in situ providing an easy statistical analysis of cell mechanical behavior. This method also requires minimum modification of widely used AFM setup, and therefore is inexpensive.

技术应用

Cell biology Tissue analysis Biosensoring

详细技术说明

Researchers at UC Davis have invented a new methodology to measure single cell mechanics. The new method utilizes atomic force microscopy (AFM) to perform single cell compression. Large sphere glued to the end of AFM probe compresses cell with independent displacement and force detection in wide range from 1 nN up to 50 µN. A high-resolution bright field or fluorescent microscopy guides the location of the probe and monitors the deformation of cell and cell organelles during the load cycle. Many previously developed methods probe only limited local deformation of membrane providing knowledge about local membrane properties. In contrast, in our scheme the indenter compresses the whole cell for 100%. In developed method whole cell compression together with optical recording of compression cycle contains information about membrane and cytoskeleton structure, integrity of cell, cell sensitivity to chemical treatment or gene over expression and cell viability. Images Schematic of developed methodic Side view schematic of developed methodic Bottom view of compressed cell. Numbers represent relative cell compression.

*Abstract

Atomic Force Microscopy-based Platform for Investigating Single Cell Mechanics

*IP Issue Date

Dec 4, 2012

*Principal Investigation

Name: Gang-yu Liu

Department:

Name: Valentyn Liulevych

Department:

附加资料

Patent Number: US8323920B2
Application Number: US2008301685A
Inventor: Liulevych, Valentyn | Liu, Gang-yu
Priority Date: 26 Jun 2006
Priority Number: US8323920B2
Application Date: 20 Nov 2008
Publication Date: 4 Dec 2012
IPC Current: C12Q000102 | C12Q000100
US Class: 435029 | 435004
Assignee Applicant: The Regents of the University of California
Title: Method and system for measuring single cell mechanics using a modified scanning probe microscope
Usefulness: Method and system for measuring single cell mechanics using a modified scanning probe microscope
Summary: The method and system are useful for measuring single cell mechanics using a scanning probe microscope.
Novelty: Measuring single cell mechanics using modified scanning probe microscope comprises extracting mechanical properties of the cell from cell deformation behavior and cell response to the compression force

主要类别

生物医学

细分类别

DNA /基因工程

申请号码

8323920

其他

Additional Technologies by these Inventors

• A New Methodology for 3D Nanoprinting

Tech ID/UC Case

11266/2006-727-0

Related Cases

2006-727-0

国家/地区

美国