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Human MUC5B Promoter-Reporter Construct

技术优势

The new MUC5B promoter-reporter construct provides the basis for a simple assay to test for compounds that regulate MUC5B expression.

技术应用

Can be used to identify compounds that regulate MUC5B expression May be useful in the development of therapeutics for airway diseases such as cystic fibrosis, chronic bronchitis, bronchial pneumonia, and asthma

详细技术说明

Mucins are a family of high molecular weight glycoproteins secreted by epithelial cells lining various organs such as the nasal cavity, esophagus, lungs, and eyes. Mucins are a major component of mucus, and serve an important role in protecting and lubricating epithelial surfaces. There are at least twelve mucin genes in humans, with mucin 5B (MUC5B) and mucin 5AC (MUC5AC) being the main components of human airway secretions. The regulation of expression of these genes, particularly MUC5B, is critical for normal airway function. Aberrant expression of the MUC5B gene is linked to excessive mucus secretion and is closely correlated with the pathogenesis of various airway diseases. For example, MUC5B is one of the major components in mucus obtained from patients suffering from cystic fibrosis and asthma. Therefore, inhibition of MUC5B gene expression has therapeutic potential for the treatment of these diseases. Researchers at the University of California have constructed and characterized a reporter construct under the control of the MUC5B promoter. This construct contains all of the regulatory elements that are involved in the regulation of MUC5B expression. In addition, the reporter gene included in this construct allows a very simple and easy assay to determine the promoter activity of MUC5B in response to various treatments and is amenable to high throughput screening. Therefore, this construct will be useful for screening drug candidates for inhibition of MUC5B expression.

*Abstract

Method for Inhibition of MUC5B Expression

*IP Issue Date

Nov 16, 2004

*Principal Investigation

Name: Yin Chen

Department:

Name: Reen Wu

Department:

附加资料

Patent Number: US6818446B2
Application Number: US2001990613A
Inventor: Wu, Reen | Chen, Yin
Priority Date: 21 Nov 2001
Priority Number: US6818446B2
Application Date: 21 Nov 2001
Publication Date: 16 Nov 2004
IPC Current: C07K001447 | C12N000508
US Class: 4353201 | 5360231 | 5360241
Assignee Applicant: The Regents of the University of California
Title: Compositions and methods for the analysis of mucin gene expression and identification of drugs having the ability to inhibit mucin gene expression
Usefulness: Compositions and methods for the analysis of mucin gene expression and identification of drugs having the ability to inhibit mucin gene expression
Summary: (I) is useful for identifying a compound capable of modulating MUC5B gene promoter activity.
Novelty: Novel MUC5B gene useful for identifying a compound capable of modulating MUC5B gene promoter activity

主要类别

生物医学

细分类别

DNA /基因工程

申请号码

6818446

其他

Related Materials

Characterization of human mucin 5B gene expression in airway epithelium and the genomic clone of the amino-terminal and 5'-flanking region. Am J Respir Cell Mol Biol. 2001 Nov;25(5):542-53.

Related Technologies

• Disease Markers: Mucin 5B Monoclonal Antibodies
• Mucin-Specific Monoclonal Antibodies

Additional Technologies by these Inventors

• Mucin-Specific Monoclonal Antibodies
• Disease Markers: Mucin 5B Monoclonal Antibodies
• Controlling Tumor Growth And Malignancy
• Suppression of Allergic Lung Inflammation and Hyperactivity

Tech ID/UC Case

11233/2002-008-0

Related Cases

2002-008-0

国家/地区

美国