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Novel Acoustic Wave (AW) Sensing Device Using Living Cells

技术优势

These cell-basedbiosensors can be ultra-sensitive in detecting any changes in cytoskeletalstructure or at cell organelle levels as it measures changes in its mass andvisco-elastic propertiesThe device is small,portable, and inexpensive and can be modified to a multi-wellformat for high throughput studies.Any kind of cells canbe used (ex. Human malignant cells for which chemotherapeutics are beingtested) in this ultra-sensitive label-free detection technology. Any situationor agent that will adversely affect the viability of a human cell can be sensed.The read out from thelive cell biosensor is in real time and changes can be detected much earliercompared to conventional methods.The device can beused in public places for environment monitoring. Using multiple kinds of cellsin the matrix can mimic a human body for the real-time detection of any toxicthreat present.

详细技术说明

A novel cell-based biosensor (AW device) with novel circuitry characterizes the mechanical as well as visco-elastic properties of the cells. Frequency-dependent response of the oscillator can be used to obtain more detailed diagnostics of the status of the cell in response to drug, toxins, pathogens etc.

*Abstract

None

*Background

Acousticwave (AW) devices are mass-sensitive transducers in which the changes occurringat the transducer surface can be followed by measuring the change in resonantbehavior using quartz crystal microbalance (QCM). Recently, cell-basedbiosensors have been developed where living cells are attached to the goldsurface of the quartz crystal and served as the intelligent integrated biologicalsensing elements. However, commercially developed AW devices with fixed crystalfrequencies are not designed or optimized for use with living cells since theyonly characterize a single mechanical states of the cells.

国家/地区

美国