A DNA-Cleaving DNAzyme-Based Fluorescent Sensor for Cu²+ Detection
- 详细技术说明
- This invention can provide highly sensitive and selective detection of Cu2+ in heavy water.
- *Abstract
-
Copper is a widely used metal that can leak into the environment through various routes. In low concentrations, copper is an essential nutrient; however, exposure to high levels of copper even for a short period of time can cause gastrointestinal disturbance. Long term exposure causes liver or kidney damage.
Most fluorescent sensors for Cu2+ detection give quenched fluorescence, because Cu2+ is a paramagnetic metal ion. These sensors are prone to false positive results because anything that can quench fluorescence could be mistakenly interpreted as Cu2+.
This new sensor can give over 13-fold enhancement in fluorescence in the presence of very low concentrations of Cu2+.
Most sensors for Cu2+ detection are based on fluorescent organic molecules that can selectively bind Cu2+. Upon Cu2+ binding, these molecules usually show quenched fluorescence. Although some of these sensors are very sensitive and selective for Cu2+, their practical applications are limited due to their “light-down” nature.
This invention can provide highly sensitive and selective detection of Cu2+ in heavy water. In this invention, Cu2+ recognition and signaling are spatially separated. Therefore, Cu2+ quenching efforts are minimized.
For more information about this technology, please contact the University of Illinois at Urbana-Champaign Office of Technology Management at otm@illinois.edu.
- *IP Issue Date
- None
- *IP Type
- Utility
- 国家
- United States
- 申请号码
- 8409800
- 国家/地区
- 美国
