Spirolactam and Related Targeting Compounds
DescriptionThis invention describes a novel tetracysteine (TC) peptide tag created by Dr. Stephen Miller, called SplAsH (SPiroLactam ArSenical Hairpin binder). Unlike currently available tags (SNAP-Tag sold by Covalys, HaloTag sold by Promega, and TC-FlAsH/TC-ReAsH sold by Invitrogen) SplAsH is small and capable of being labeled by a broad array of fluorescent molecules. Furthermore, the SplAsH targeting strategy overcomes current limitations with TC peptide tags in that it is more photostabile, has a longer-wavelength emission, and brighter fluorescence. Application• Fluorescent imaging of proteins with small molecules in vitro.• Labeling proteins in living cells.Advantage This invention improves upon current commercially available products by permitting the labeling of the very small TC peptide tag with a much broader array of fluorophores, allowing the use of dyes with much greater resistance to photobleaching, longer-wavelength emission, and brighter fluorescence.
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美国
