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High Throughput Pipeline for Nanobody Production

详细技术说明

Nanobodies are single domain antibodies derived from the variable regions of camelid atypical immunoglobulins. Due to their small size, nanobodies can be easily genetically manipulated and produced in large quantities with simple bacterial expression systems. Nanobodies are also extremely stable and bind antigens with very high affinity. Therefore, nanobodies represent promising reagents for research, diagnostic and therapeutic purposes. However, until recently no rapid and robust techniques have been discovered for the production of large amounts of nanobodies.

*Abstract

Our researchers have developed a rapid and low cost approach for the production of large repertoires of high affinity and specific nanobodies against a given antigen. To prove the efficacy of their approach, they were able to produce a number of nanobodies raised specifically against GFP, YFP, mCherry, etc. Their method allows the production and the generation of a large antibody repertoire against multiple epitopes in a chosen antigen. Additionally, through the use of mass spectrometry, this approach is able to identify nanobody sequences directly the animal serum. The versatility and potential of nanobodies is very high in several application fields, including drug development and cancer diagnostics.

国家/地区

美国