Gene Targeting Using VirE2 Mutant Agrobacterium Strains
- 总结
- Researchers at Purdue University have developed a unique technology of gene targeting by introducing T-DNA into the plant in the absence of VirE2 protein. It will provide a method of efficient gene targeting without building cumbersome protein scaffold systems. It will also likely have fewer off-target effects.
- 技术优势
- Efficient gene targeting Fewer off-targets
- 技术应用
- Biological identification Genetic markers Gene replacement Genome editing
- 详细技术说明
- Stanton GelvinPurdue Biological Sciences
- *Abstract
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- *Background
- Agrobacterium-mediated plant transformation is the core technology for introducing genes into plants. Usually, transferred DNA (T-DNA) is integrated into the plant genome in order to stably transform plants. Scientists now wish to use Agrobacterium to deliver DNA that will target plant genes for specific use as gene transfer technology has become more sophisticated. However, this has previously not been possible at a usable frequency. Scientists have also tried to target specific genes; however, off-targeting, which is targeting of related but not identical genes, is a major problem. These methods are cumbersome and require building complex proteins for targeting.
- *IP Issue Date
- None
- *IP Type
- Utility
- *Stage of Development
- Prototype testing
- *Web Links
- Purdue Office of Technology CommercializationPurdueInnovation and EntrepreneurshipStanton GelvinPurdue Biological Sciences
- 国家
- United States
- 申请号码
- None
- 国家/地区
- 美国
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