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"DiGEP" Technology for Phosphoprotein Analysis

总结

To resolve these issues, researchers at Purdue University have developed a novel gel-based process, called Differential Gel Electrophoresis of Phosphoproteome (DiGEP). This process uses metal ions, such as Ti4+, Zr4+, Fe3+, and Ga3+, which are attracted to phosphate groups and attach fluorophores on the phosphoproteins. This ultimately helps visualize phosphorylation changes in different samples on the same gel. DiGEP analyses are advantageous because they enable the visualization of phosphoproteins on a single gel and select only relevant proteins for in-gel digestion and mass spectrometric analysis. It is highly specific, selective, and quantitative and can be used routinely in labs for quantitative phosphorylation measurement, in vitro kinase assay, kinase and phosphatase activity assay, kinase/phosphatase inhibitor screening, and detection of in vivo phosphorylation. Not only will this technology help a general biological laboratory to effectively measure changes in protein phosphorylation, but will also help pharmaceutical and biotech industries to develop effective diagnostic and therapeutic agents relating to kinases.

技术优势

Visualization of phosphoproteinsSelection of proteins for spectrometric analysis Effective and quantitative measures

技术应用

Research & Development LabsDisease Research

详细技术说明

W. Andy Tao - BiochemistryW. Andy Tao - ChemistryW. Andy Tao - Medicinal Chemistry and Molecular PharmacologyTao Research GroupPurdue BiochemistryPurdue ChemistryPurdue Medicinal Chemistry and Molecular Pharmacology

*Abstract

*Background

Research and Development labs (R&D) in academia, pharmaceutical, and biotech companies often assess the phosphorylation of a protein or classes of proteins. Currently, large-scale phosphorylation analysis is used to understand how signaling pathways work and how they may be deregulated in disease states. Even though thousands of protein phosphorylation sites can be identified, researchers may only be interested in a small sample of proteins for a signaling pathway. Hence, these large scale analysis techniques are time-consuming and cost-prohibitive. The applicability of gel-based proteomic strategy in phosphoproteomics has been largely limited by the lack of technologies for specific and quantitative detection of phosphoproteins in gels.

*IP Issue Date

None

*IP Type

Provisional

*Stage of Development

Process Validation in Lab

*Web Links

Purdue Office of Technology CommercializationPurdue Innovation and EntrepreneurshipW. Andy Tao - BiochemistryW. Andy Tao - ChemistryW. Andy Tao - Medicinal Chemistry and Molecular PharmacologyTao Research GroupPurdue BiochemistryPurdue ChemistryPurdue Medicinal Chemistry and Molecular Pharmacology

国家

United States

申请号码

None

国家/地区

美国