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High Throughput Quantitative Proteomics

总结

Researchers at Purdue University have developed methods that allow for the mutual presence of positively charged and negatively charged ions such that the ions can react with one another and whereby the kinetics of reaction of ions of selected mass-to-charge ratios can be reduced relative to those of non-selected ions. This capability allows for highly charged ions derived from a particular high mass molecule, usually formed via electrospray ionization, to be accumulated into a selected charge state. Furthermore, this capability allows for ions present in a complex mixture of ions of like polarity to be selectively removed from the population of ions undergoing ion/ion reactions. This capability is particularly useful for the analysis of complex mixtures of high mass molecules. Such an overall capability would be attractive, for example, in proteomics applications.

技术优势

Provides means for accumulating ions into predetermined charge states for isolating species from complex mixtures of ionsIsolates one or more ions of defined mass-to-charge ratio(s) and minimizes the extent to which the isolated ions undergo ion/ion reactions in the presence of ions of opposite polarity

技术应用

Chemical AnalysisMass SpectrometryAnalytical Methods

详细技术说明

Fred RegnierPurdue Chemistry

*Abstract

*Background

Ion/ion reaction technologies that are used to manipulate ion charge state distributions in mass spectrometers have been limited to product ion distributions that are determined solely by the kinetics of reaction. This limitation of product ion distributions results in the inability to accumulate ions in an arbitrary charge state while ions are undergoing ion/ion reactions.

*IP Issue Date

Mar 8, 2011

*IP Type

Continuation

*Stage of Development

Process Validation in Lab

*Web Links

Purdue Office of Technology CommercializationPurdueInnovation and EntrepreneurshipFred RegnierPurdue Chemistry

国家

United States

申请号码

7,901,943

国家/地区

美国