Assay Method Using Film Immobilized Particles
- 技术优势
- • Ability to optimize solid phase and coating conditions • Diminished non-specific binding • Compatible with a wide range of buffers with various pHs and ionic strengths • Ability to use a variety of capture particles
- 详细技术说明
- Novel method for performing and analyzing biological interactions utilizing a thin film of immobilized capture particles. #researchtool #screening #detection #reagent
- *Abstract
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Northwestern researchers have developed a new method of performing and analyzing protein-protein interactions, immuno-hybridization, and nucleic hybridization. This method utilizes capture particles that are immobilized as a thin film coating on a planar substrate. Either a homogeneous or heterogeneous set of particles may be arranged as an array. The analyte of interest is incubated with the array, and binding is subsequently visualized and quantified using an optical detection system. The system offers several advantages. The solid phases and coating conditions for the planar substrate can be optimized depending on the characteristics of the capture reagent. The method also allows the use of commercially available particles with numerous surfaces for passive adsorption or covalent coupling to the film. Furthermore, reagents can be attached to the capture particles in a wide range of buffers with various pHs and ionic strengths, which can improve the density, kinetic rate constant, and equilibrium affinity constant of the capture reagent. Finally, non-specific binding can be minimized by pre-coating the substrate surface with blockers. Further, the capture reagents are attached to the particles with minimal changes to their binding sites.
- *Inventors
- David Kelso
- 国家/地区
- 美国
