Proteomics Sample Preparation Method
The use of this technology provides extremely accurate measurements of proteins by minimizing procedural errors due to unstable labeling of peptides to be measured. With improved peptide labeling as described in this technology, mass spectormetry-based proteomic analysis circumvents running laborious 2-dimensional gels and offers wide dynamic ranges.
The protein sample preparation technology can be used to develop a protein labeling kit as a simple and dependable comparative proteomics tool.
To achieve consistent labeling of samples for MS analysis, the number of isotopic atoms, or ΓÇ£labels,ΓÇØ to be incorporated into peptides needs to be regulated. Our new technology takes advantage of the innate property of peptidases and controls the enzymatic condition so that a user can specify the number of 18O atoms to be incorporated into sample peptides: either only one or only two, but not mixture of both. Controlling the number of isotopic atoms on sample peptides avoids miscalculation of protein amounts. This technology can be applied for the development of a sample preparation kit to make MS-based comparative proteomics simple and reliable for a research or diagnostic use.
Inventor: KUMANA C R | KWONG Y
Priority Number: CN1723029A
IPC Current: A61K003336 | A61P003502
Assignee Applicant: The University of Hong Kong
Title: Formulation of oral compositions comprising arsenic trioxide and methods of use thereof | Preparation of oral composition comprising arsenic trioxide and using method thereof
Usefulness: Formulation of oral compositions comprising arsenic trioxide and methods of use thereof | Preparation of oral composition comprising arsenic trioxide and using method thereof
Summary: Composition (A) is useful for the treatment of hematological malignancies (preferably acute myeloid leukemia (preferred), acute nonlymphocytic leukemia, myeloblastic leukemia, promyelocytic leukemia, myelomonocytic leukemia, monocytic leukemia, erythroleukemia, myelodysplastic syndrome, acute promyelocytic leukemia (preferred), chronic lymphocytic leukemia, chronic myeloid leukemia, hairy cell leukemia, polycythemia vera, Hodgkin's lymphoma, non-Hodgkin's lymphomas, myeloma, giant cell myeloma, indolent myeloma, localized myeloma, multiple myeloma, plasma cell myeloma, sclerosing myeloma, solitary myeloma, smoldering multiple myeloma, nonsecretary myeloma, osteosclerotic myeloma, plasma cell leukemia, solitary plasmacytoma, and extramedullary plasmacytoma). (all claimed)
Novelty: Preparation of oral composition, useful to treat hematological malignancies, comprises addition of sterile water to arsenic trioxide, followed by subsequent addition of sodium hydroxide and hydrochloric acid to form a solution
诊断/治疗
癌症/肿瘤
美国
