Luminescent protein Staining
The present invention relates to the use of cyclometalated iridium complexes for detecting poly(amino acids) including peptides, polypeptides, and proteins. Poly(amino acids) are detected in solution, in electrophorectic gels, and on solid supports, including blots. The method of the present invention is rapid, highly sensitive, and extremely facile.
Patent Number: US8318498B2
Application Number: US2007907011A
Inventor: Wong, Kwok-Yin | Ma, Dik-Lung | Wong, Wing-Leung | Chung, Wai-Hong | Chan, Fung-Yi
Priority Date: 9 Oct 2007
Priority Number: US8318498B2
Application Date: 9 Oct 2007
Publication Date: 27 Nov 2012
IPC Current: G01N003368 | G01N003500
US Class: 436043 | 422050 | 422504 | 436086 | 436088 | 436164 | 436167
Assignee Applicant: The Hong Kong Polytechnic University
Title: Luminescent protein staining
Usefulness: Luminescent protein staining
Summary: For detecting poly(amino acid) in a mixture (claimed), in a solution, in an electrophorectic gels, and on solid supports, including blots; for staining and for detection of biological important molecules including peptides, polypeptides and proteins luminescence in a mixture.
Novelty: Detecting poly(amino acid) in a mixture involves combining mixture with staining mixture of cyclometalated iridium complex; incubating mixture; illuminating mixture if poly(amino acid) is present; and observing detectable optical response
生物医学
医学影像
WONG Kwok-Yin
MA Dik-Lung
WONG Wing-Leung
CHUNG Wai-Hong
CHAN Fung-Yi
美国
