Stable Heterologous Propagation Of Cftr Protein Variant Cdna
A Modified Dna Sequence Encoding Full Length Cystic Fibrosis Transmembrane Conductance Regulator Protein Is Provided To Facilitate Propagation And/Or Expression Of The Protein In Living Cells And In Particular, Bacterial Cells. The Modified Dna Sequence Comprises At Least One Of The 13 Base Pair Repeat Of Exon 6B Of The Normal Gene Encoding The Conductance Regulator Protein, As One Or More Normal Nucleotides Of The 13 Base Repeat Substituted With An Alternate Nucleotide Which, However, Continues To Code For The Corresponding Normal Amino Acid. Mammalian Cells Transferred With A Vector Containing The Modified Dna Sequence Enhances Chlorine Conductance Through The Cell Wall
Inventor: Tsui, Lap Chee | Rommens, Johanna M.
Priority Number: US5863770A
IPC Current: C07K001447 | C07K001618 | C07K001628 | C12N001585 | A61K004800
US Class: 4350712 | 4350691 | 4350701 | 4350711 | 4350914 | 4352523 | 4353201 | 435366 | 4351723 | 935023 | 935071 | 935072 | 935070
Assignee Applicant: HSC Research and Development Limited Partnership,Toronto
Title: Stable heterologous propagation of CFTR protein variant Cdna | Stable meterologous propagation of CFTR protein variant cDNA
Usefulness: Stable heterologous propagation of CFTR protein variant Cdna | Stable meterologous propagation of CFTR protein variant cDNA
Novelty: Modified DNA sequence derived from gene coding for cystic fibrosis trans:membrane conductance regulator protein
生物医学
DNA /基因工程
香港
