STABLE PROPAGATION OF MODIFIED FULL LENGTH CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR PROTEIN cDNA IN HETEROLOGOUS SYSTEMS
A modified DNA sequence encoding full length cystic fibrosis transmembrane conductance regulator protein is provided to facilitate propagation and/or expression of the protein in living cells and in particular, bacterial cells. The modified DNA sequence comprises at least one of the 13 base pair repeat of exon 6b of the normal gene encoding the conductance regulator protein, as one or more normal nucleotides of the 13 base pair repeat substituted with an alternate nucleotide which however continues to code for the corresponding normal amino acid. Mammalian cells transfected with a vector containing the modified DNA sequence enhances chlorine conductance through the cell wall.
Patent Number: AU199185221A
Application Number: AU199185221D
Inventor: TSUI LAP-CHEE | ROMMENS JOHANNA M
Priority Date: 21 Sep 1990
Priority Number: AU199185221A
Application Date: 20 Sep 1991
Publication Date: 15 Apr 1992
IPC Current: C07K001447 | C12N000121 | C12N001512
Assignee Applicant: HSC RES DEV LP
Title: STABLE PROPAGATION OF MODIFIED FULL LENGTH CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR PROTEIN CDNA IN HETEROLOGOUS SYSTEMS
Usefulness: STABLE PROPAGATION OF MODIFIED FULL LENGTH CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR PROTEIN CDNA IN HETEROLOGOUS SYSTEMS
Novelty: Modified DNA sequence derived from gene coding for cystic fibrosis trans:membrane conductance regulator protein
生物医学
DNA /基因工程
香港
