Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids
Methods are disclosed herein for inducing homologous recombination in a host cell comprising a target nucleic acid, using a single-stranded nucleic acid molecule. The single-stranded nucleic acid molecule has a sufficient number of nucleotides homologous to the target nucleic acid to enable homologous recombination with the target nucleic acid. The host cell includes a de-repressible promoter operably linked to a nucleic acid encoding a single-stranded binding protein and is deficient for mismatch repair. Isolated host cells of use in this method are also disclosed.
Patent Number: US7521242B2
Application Number: US2004841125A
Inventor: Court, Donald L. | Li, Xin-tian | Huang, Jian-Dong | Costantino, Nina | Liu, Depei
Priority Date: 9 May 2003
Priority Number: US7521242B2
Application Date: 7 May 2004
Publication Date: 21 Apr 2009
IPC Current: C12N001587 | C12N000120 | C12N001570 | C12N001590 | C12N000121
US Class: 435490 | 43525233 | 435471
Assignee Applicant: The United States of America as represented by the Department of Health and Human Servicesshington
Title: Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids
Usefulness: Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids
Summary: (M1) is useful for inducing homologous recombination in a host cell having a target nucleic acid and deficient for mismatch repair (claimed).
Novelty: Inducing homologous recombination in host cell having target nucleic acid, by introducing nucleic acid molecule with nucleotides homologous to target, into host cell, inducing expression of single-stranded binding protein in host cell
生物医学
DNA /基因工程
香港
