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A Cost-effective Method for Expression and Purification of Recombinant Proteins in Plants

总结

Downstream purification of recombinant proteins from plant-based samples is performed by Elastin-like polypeptides (ELP)-intein fusion expression and purification through temperature-sensitive inverse phase transition of ELP and cleavage reaction of intein.

附加资料

Patent Number: US20120184714A1
Application Number: US13333830A
Inventor: SUN, Samuel Sai Ming | Tian, Li
Priority Date: 21 Dec 2010
Priority Number: US20120184714A1
Application Date: 21 Dec 2011
Publication Date: 19 Jul 2012
IPC Current: C07K001900 | A01H000500 | C07K000114 | C12N000510 | C12N001562 | C12P002100
US Class: 530353 | 4350697 | 435419 | 5360234 | 800298
Assignee Applicant: The Chinese University of Hong Kong
Title: COST-EFFECTIVE METHOD FOR EXPRESSION AND PURIFICATION OF RECOMBINANT PROTEINS IN PLANTS
Usefulness: COST-EFFECTIVE METHOD FOR EXPRESSION AND PURIFICATION OF RECOMBINANT PROTEINS IN PLANTS
Summary: For obtaining desired protein from plants, plant parts, or plant cells; where desired protein is lectin or cytokine; and for producing fusion protein having elastin-like polypeptide (ELP)-intein tag (claimed). Also useful for producing recombinant proteins such as pharmaceuticals, antibody chains, industrial enzymes, and other useful proteins.
Novelty: Obtaining protein from plants involves forming protein extract from plant having fusion protein having at terminal(s) elastin-like polypeptide and splicing intein tag, aggregating, resolubilizing, cleaving and separating protein from tag(s)

主要类别

化工/材料

细分类别

化工/材料应用

申请号码

10/SCI/400/ITF

其他

Inventor(s): Professor Samuel SUN, School of Life Sciences

Patent Status: US Patent Pending

Licensing Status: Available for licensing

国家/地区

香港