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Monoclonal Antibody Panel Against Glycoproteins G1 and G2 of Tomato Spotted Wilt Virus (TSWV, Tospovirus) (8 lines)

Detailed Technology Description

Applications Suitable for DAS-ELISA, PAS-ELISA and DIBA applications.Tomato spotted wilt tospovirus (TSWV) is the type member ofthe plant-infecting viruses of the genus Tospovirus in the family Bunyaviridae.TSWV is transmitted by thrips (Thysanoptera: Thripidae) to over 650 differentplant species in temperate and tropical regions. Once the plants infected withthe virus, they result in spotting, wilting, reduced vegetative output anddeath of the plant. Some of the crops known to be affected by the virusesinclude, peanuts, watermelons, tomatoes, zucchinis and others.

*Abstract

ReagentsDescription

 

Clone Name	Antigen	Immunogen	MW (KDa)	Reactivity/Recognition	Tested Applications
G1-5D5.G5	G1 glycoprotein of TSWV	Glycoprotein	95	TSWV, possibly other Tospoviruses or Bunyaviridae	ELISA, WB, IF
5F11	G2 Glycoprotein of TSWV	Glycoprotein	58	TSWV, possibly other Tospoviruses or Bunyaviridae	ELISA, WB, IF
6G8	G2 Glycoprotein of TSWV	Glycoprotein	58	TSWV, possibly other Tospoviruses or Bunyaviridae	ELISA, WB, IF
G2.1D6.H11	G2 Glycoprotein of TSWV	Glycoprotein	58	TSWV, possibly other Tospoviruses or Bunyaviridae	ELISA, WB, IF
G2	G2 Glycoprotein of TSWV	Glycoprotein	58	TSWV, possibly other Tospoviruses or Bunyaviridae	ELISA, WB, IF, IB
G2-1G6.A8	G2 Glycoprotein of TSWV	Glycoprotein	58	TSWV, possibly other Tospoviruses or Bunyaviridae	ELISA, WB, IF, IB
G2-6D1.KC6	G2 Glycoprotein of TSWV	Glycoprotein	58	TSWV, possibly other Tospoviruses or Bunyaviridae	ELISA, WB, IF
HL-1.G2	G2 Glycoprotein of TSWV	Glycoprotein	58	TSWV, possibly other Tospoviruses or Bunyaviridae	ELISA, WB, IF

SpeciesImmunized:  Mouse

Purification Method:  Unpurified. Used as supernatant

Antibodystorage temperature: -80^oC

References

 

Sherwood, JL,Sanborn, MR, Keyser, GC; Myers, LD. Use of Monoclonal Antibodies in Detectionof Tomato Spotted Wilt Virus, Phytopathology, 79(1),61-64 (1989).

Bandla, MD,Daphne, MW; Chenault, K.D.; Ullman, DE; German, TL; Sherwood, JL: Use ofMonoclonal Antibody to the Nonstructural Protein Encoded by the Small RNA ofTomato Spotted Wilt Tospovirus to Identify Viruliferous Thirps: Phytopathology,84(12), 1427-1431 (1994).

Naidu, RA;Sherwood, JL; Deom, CM. Characterization of a vector-non-transmissible isolateof Tomato spotted wilt virus. Plant Pathol,57, 190-200 (2008).

Richmond, KE,Chenault, K; Sherwood, JL; Geramn, TL: Characterization of the Nucleic AcidBinding Properties of Tomato Spotted Wilt Virus Nucleocapsid Protein, Virology 248,6-11 (1998).

Adkins, S, Choi,T-J,Israel, BA, Bandla, MD, Richmond, KE, Schultz, KT, Sherwood, JL, German,TL. Baculovirus Expression and Processing of Tomato Spotted Wilt TopovirusGlycoproteins. Phytopathology86(8), 849-855 (1996).

Country/Region

USA